A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

• Main Authors: Ho, Nurulain, Yap, Wei Sheng, Thibault, Guillaume
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; High Throughput Screening; Cell-based Assays
• Online Access: https://hdl.handle.net/10356/164053
• Institution: Nanyang Technological University

The endoplasmic reticulum (ER) stress is defined by the accumulation of unfolded proteins at the ER and perturbation at the ER membrane, known as lipid bilayer stress (LBS). In turn, ER stress triggers the unfolded protein response (UPR) to restore ER homeostasis. Here, we provide a modified protoco...