A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

• المؤلفون الرئيسيون: Ho, Nurulain, Yap, Wei Sheng, Thibault, Guillaume
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2023
• الموضوعات: Science::Biological sciences; High Throughput Screening; Cell-based Assays
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/164053

The endoplasmic reticulum (ER) stress is defined by the accumulation of unfolded proteins at the ER and perturbation at the ER membrane, known as lipid bilayer stress (LBS). In turn, ER stress triggers the unfolded protein response (UPR) to restore ER homeostasis. Here, we provide a modified protoco...