Protein-mediated fluorescence resonance energy transfer (P-FRET) probe: fabrication and hydroxyl radical detection

• Main Authors: Yu, Xiaokan, Zhu, Weisheng, Ouyang, Wenao, Zhang, Xiaojia, Qiu, Hao, Zhang, Zhijun, Xing, Bengang
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Chemistry; Bovine Serum-Albumin; Ratiometric Detection
• Online Access: https://hdl.handle.net/10356/164113
• Institution: Nanyang Technological University

Fluorescent probes based on fluorescence resonance energy transfer (FRET) are highly promising for diverse bioapplications. The key to constructing FRET probes is to confine the donor and acceptor within a sufficiently close distance. However, the commonly used covalent linkage often requires elabor...