Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)

Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source (Tang et al., 2011). Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimi...

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Main Authors: Armstrong, Michael D. Sorochan, Campos, O. René Arredondo, Bannon, Catherine C., de la Mata, A. Paulina, Case, Rebecca J., Harynuk, James J.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164118
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1641182023-01-05T04:00:04Z Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS) Armstrong, Michael D. Sorochan Campos, O. René Arredondo Bannon, Catherine C. de la Mata, A. Paulina Case, Rebecca J. Harynuk, James J. School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering Science::Biological sciences Dunaliella Tertiolecta Dunaliellaceae Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source (Tang et al., 2011). Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimised growth conditions, and more recently, algal-bacterial co-cultures have been explored as a way of improving the yield of D. tertiolecta microalgae oils. The relationship between the host microalgae and bacterial co-cultures is currently poorly understood. Here, a complete workflow is proposed to analyse the global metabolomic profile of co-cultured D. tertiolectra and Phaeobacter italicus R11, which will enable researchers to explore the chemical nature of this relationship in more detail. To the best of the authors' knowledge this study is one of the first of its kind, in which a pipeline for an entirely untargeted analysis of the algal metabolome is proposed using a practical sample preparation, introduction, and data analysis routine. Agency for Science, Technology and Research (A*STAR) The authors wish to acknowledge the support of the Natural Sciences and Engineering Research Council of Canada (NSERC) and the support given to The Metabolomics Innovation Centre (TMIC) through grants from Genome Alberta, Genome Canada, and The Canada Foundation for Innovation. This work was also supported by A*STAR SFS IAF-PP grant (A20H7a0152) awarded to Rebecca Case. 2023-01-05T04:00:04Z 2023-01-05T04:00:04Z 2022 Journal Article Armstrong, M. D. S., Campos, O. R. A., Bannon, C. C., de la Mata, A. P., Case, R. J. & Harynuk, J. J. (2022). Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS). Phytochemistry, 195, 113052-. https://dx.doi.org/10.1016/j.phytochem.2021.113052 0031-9422 https://hdl.handle.net/10356/164118 10.1016/j.phytochem.2021.113052 34968885 2-s2.0-85121979786 195 113052 en A20H7a0152 Phytochemistry © 2021 Elsevier Ltd. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Dunaliella Tertiolecta
Dunaliellaceae
spellingShingle Science::Biological sciences
Dunaliella Tertiolecta
Dunaliellaceae
Armstrong, Michael D. Sorochan
Campos, O. René Arredondo
Bannon, Catherine C.
de la Mata, A. Paulina
Case, Rebecca J.
Harynuk, James J.
Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
description Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source (Tang et al., 2011). Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimised growth conditions, and more recently, algal-bacterial co-cultures have been explored as a way of improving the yield of D. tertiolecta microalgae oils. The relationship between the host microalgae and bacterial co-cultures is currently poorly understood. Here, a complete workflow is proposed to analyse the global metabolomic profile of co-cultured D. tertiolectra and Phaeobacter italicus R11, which will enable researchers to explore the chemical nature of this relationship in more detail. To the best of the authors' knowledge this study is one of the first of its kind, in which a pipeline for an entirely untargeted analysis of the algal metabolome is proposed using a practical sample preparation, introduction, and data analysis routine.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Armstrong, Michael D. Sorochan
Campos, O. René Arredondo
Bannon, Catherine C.
de la Mata, A. Paulina
Case, Rebecca J.
Harynuk, James J.
format Article
author Armstrong, Michael D. Sorochan
Campos, O. René Arredondo
Bannon, Catherine C.
de la Mata, A. Paulina
Case, Rebecca J.
Harynuk, James J.
author_sort Armstrong, Michael D. Sorochan
title Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
title_short Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
title_full Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
title_fullStr Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
title_full_unstemmed Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
title_sort global metabolome analysis of dunaliella tertiolecta, phaeobacter italicus r11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (td-gc×gc-tofms)
publishDate 2023
url https://hdl.handle.net/10356/164118
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