Effect of temperature decrease on anammox granular sludge: shock and adaptation

Cryopreservation is one of the effective methods for the preservation of anammox granular sludge (AnGS). However, the effects of cooling pretreatment on AnGS are still unclear. In this study, the effects of temperature decrease on AnGS property were investigated by designing different cooling modes:...

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Main Authors: Pan, Chao, Xu, Dongdong, Dong, Ziyang, Li, Wenji, Kang, Da, Chen, Wenda, Li, Yiyu, Zhang, Meng, Hu, Baolan, Zheng, Ping
Other Authors: Nanyang Environment and Water Research Institute
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164191
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1641912023-01-09T04:21:00Z Effect of temperature decrease on anammox granular sludge: shock and adaptation Pan, Chao Xu, Dongdong Dong, Ziyang Li, Wenji Kang, Da Chen, Wenda Li, Yiyu Zhang, Meng Hu, Baolan Zheng, Ping Nanyang Environment and Water Research Institute Advanced Environmental Biotechnology Centre (AEBC) Engineering::Environmental engineering Anammox Anammox Granular Sludge Cryopreservation is one of the effective methods for the preservation of anammox granular sludge (AnGS). However, the effects of cooling pretreatment on AnGS are still unclear. In this study, the effects of temperature decrease on AnGS property were investigated by designing different cooling modes: constant at room temperature 20-25 °C (CK), sharp cooling to 4 °C (S4), -20 °C (S20) and stepwise cooling to 4 °C (A4), -20 °C (A20). The results showed that compared with CK, the cooling modes in S4, S20, A4 and A20 improved the physical preservability of AnGS, slowing down the changes of color, shape and structure; and elevated the preservation rate of functional bacteria Planctomycetes (phylum level) and Candidatus Brocadia (genus level). The preservation rate of live cells in different experimental groups was 48.4 ± 1.8%(CK), 61.1 ± 3.3%(S4), 37.8 ± 0.8%(S20), 81.7 ± 4.8%(A4), 61.9 ± 3.1%(A20), respectively. The Anaerobic Ammonium Oxidation Bacteria (AnAOB) in the stepwise cooling mode (A4 and A20) were found to enter the dormant state and form "dormant zoogloea", while the AnAOB in the sharp cooling mode (S4 and S20) were observed to enter the shock state with a little change. The findings in this work (especially the dormant state of AnAOB) are helpful to understand the effect of temperature decrease on AnGS and to promote the development of AnGS preservation technology. The authors are grateful for financial support from the National Natural Science Foundation of China (52070163, 51778563). 2023-01-09T04:21:00Z 2023-01-09T04:21:00Z 2021 Journal Article Pan, C., Xu, D., Dong, Z., Li, W., Kang, D., Chen, W., Li, Y., Zhang, M., Hu, B. & Zheng, P. (2021). Effect of temperature decrease on anammox granular sludge: shock and adaptation. Science of the Total Environment, 798, 149242-. https://dx.doi.org/10.1016/j.scitotenv.2021.149242 0048-9697 https://hdl.handle.net/10356/164191 10.1016/j.scitotenv.2021.149242 34375252 2-s2.0-85111546076 798 149242 en Science of the Total Environment © 2021 Elsevier B.V. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Anammox
Anammox Granular Sludge
spellingShingle Engineering::Environmental engineering
Anammox
Anammox Granular Sludge
Pan, Chao
Xu, Dongdong
Dong, Ziyang
Li, Wenji
Kang, Da
Chen, Wenda
Li, Yiyu
Zhang, Meng
Hu, Baolan
Zheng, Ping
Effect of temperature decrease on anammox granular sludge: shock and adaptation
description Cryopreservation is one of the effective methods for the preservation of anammox granular sludge (AnGS). However, the effects of cooling pretreatment on AnGS are still unclear. In this study, the effects of temperature decrease on AnGS property were investigated by designing different cooling modes: constant at room temperature 20-25 °C (CK), sharp cooling to 4 °C (S4), -20 °C (S20) and stepwise cooling to 4 °C (A4), -20 °C (A20). The results showed that compared with CK, the cooling modes in S4, S20, A4 and A20 improved the physical preservability of AnGS, slowing down the changes of color, shape and structure; and elevated the preservation rate of functional bacteria Planctomycetes (phylum level) and Candidatus Brocadia (genus level). The preservation rate of live cells in different experimental groups was 48.4 ± 1.8%(CK), 61.1 ± 3.3%(S4), 37.8 ± 0.8%(S20), 81.7 ± 4.8%(A4), 61.9 ± 3.1%(A20), respectively. The Anaerobic Ammonium Oxidation Bacteria (AnAOB) in the stepwise cooling mode (A4 and A20) were found to enter the dormant state and form "dormant zoogloea", while the AnAOB in the sharp cooling mode (S4 and S20) were observed to enter the shock state with a little change. The findings in this work (especially the dormant state of AnAOB) are helpful to understand the effect of temperature decrease on AnGS and to promote the development of AnGS preservation technology.
author2 Nanyang Environment and Water Research Institute
author_facet Nanyang Environment and Water Research Institute
Pan, Chao
Xu, Dongdong
Dong, Ziyang
Li, Wenji
Kang, Da
Chen, Wenda
Li, Yiyu
Zhang, Meng
Hu, Baolan
Zheng, Ping
format Article
author Pan, Chao
Xu, Dongdong
Dong, Ziyang
Li, Wenji
Kang, Da
Chen, Wenda
Li, Yiyu
Zhang, Meng
Hu, Baolan
Zheng, Ping
author_sort Pan, Chao
title Effect of temperature decrease on anammox granular sludge: shock and adaptation
title_short Effect of temperature decrease on anammox granular sludge: shock and adaptation
title_full Effect of temperature decrease on anammox granular sludge: shock and adaptation
title_fullStr Effect of temperature decrease on anammox granular sludge: shock and adaptation
title_full_unstemmed Effect of temperature decrease on anammox granular sludge: shock and adaptation
title_sort effect of temperature decrease on anammox granular sludge: shock and adaptation
publishDate 2023
url https://hdl.handle.net/10356/164191
_version_ 1754611271503708160