Mechanotransduction of cells under physical constraints
In this report, a direct quantitative way to measure the mechanotransduction of cells under physical constraints was discussed. This was accomplished by both microcontact printing and cell traction force microscopy study. A flexible polyacrylamide (PAL) gel embedded with fluorescent microbeads wa...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/16448 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | In this report, a direct quantitative way to measure the mechanotransduction of cells under
physical constraints was discussed. This was accomplished by both microcontact printing and
cell traction force microscopy study. A flexible polyacrylamide (PAL) gel embedded with
fluorescent microbeads was polymerized on an activated glass surface. Adhesive islands were
created on the surface of the gel or directly on activated glass surfaces by microcontact printing.
Rat aorta cells were cultured on these islands. The cells spread to take on the shape and size of
the islands. Cell traction force was then quantified by mapping displacement fields of the
fluorescent microbeads within the micron-sized adhesive islands of defined shape and size. The
results drew the relationship between cell traction force and various cell shapes. |
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