Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye

The ocular surface microbiome has implications for ocular surface inflammation and immunology. Previous shotgun metagenomics analyses were performed in China, showing results that differed according to environment and age. Patients with Sjogren's syndrome were reported to have altered conjuncti...

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Main Authors: Tong, Louis, Constancias, Florentin, Hou, Aihua, Chua, Song Lin, Drautz-Moses, Daniela Isabel, Schuster, Stephan Christoph, Yang, Liang, Williams, Rohan B. H., Kjelleberg, Staffan
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164505
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spelling sg-ntu-dr.10356-1645052023-02-04T23:34:46Z Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye Tong, Louis Constancias, Florentin Hou, Aihua Chua, Song Lin Drautz-Moses, Daniela Isabel Schuster, Stephan Christoph Yang, Liang Williams, Rohan B. H. Kjelleberg, Staffan Lee Kong Chian School of Medicine (LKCMedicine) School of Biological Sciences Life Sciences Institute, NUS Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Science::Medicine Human Microbiome Inflammation The ocular surface microbiome has implications for ocular surface inflammation and immunology. Previous shotgun metagenomics analyses were performed in China, showing results that differed according to environment and age. Patients with Sjogren's syndrome were reported to have altered conjunctival microbiome, but such studies have not been done in milder dry eye. The aim of this study is to describe the conjunctival microbiome in people with mild dry eye in Singapore. Samples were collected from 14 participants with mild dry eye and 10 age-matched comparison participants recruited from Singapore National Eye Centre (SNEC) clinics. Shotgun metagenomic sequencing analysis was employed to evaluate the conjunctival microbiome composition. Proteobacteria formed the predominant phylum in the conjunctiva. As in a study from a coastal city in China, Achromobacter spp. was numerically most abundant. Compared to age-matched controls, the conjunctival microbial composition in mild dry eye was similar. Several microorganisms, including Streptococcus spp. increased in representation with age, and the abundance of Staphylococcus correlated with Schirmer readings. In addition, when cultured corneal epithelial cells were exposed to three strains of Achromobacter xylosoxidans, cytokines such as TNF-α and IL-6 were upregulated in the cell lysates and supernatants. Ourresults suggest that age is an important factor that affects composition of the conjunctival microbiome, and relative abundance of specific microorganism may vary according to the environment of the human host. National Medical Research Council (NMRC) Published version This research was supported by the National Medical Research Council (NMRC) (NMRC/CSA/045/2012 and NMRC/CSA-SI/0017/2017). 2023-01-30T04:18:55Z 2023-01-30T04:18:55Z 2022 Journal Article Tong, L., Constancias, F., Hou, A., Chua, S. L., Drautz-Moses, D. I., Schuster, S. C., Yang, L., Williams, R. B. H. & Kjelleberg, S. (2022). Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye. Frontiers in Medicine, 9, 1034131-. https://dx.doi.org/10.3389/fmed.2022.1034131 2296-858X https://hdl.handle.net/10356/164505 10.3389/fmed.2022.1034131 36438051 2-s2.0-85142642341 9 1034131 en NMRC/CSA/045/2012 NMRC/CSA-SI/0017/2017 Frontiers in Medicine © 2022 Tong, Constancias, Hou, Chua, Drautz-Moses, Schuster, Yang, Williams and Kjelleberg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Human Microbiome
Inflammation
spellingShingle Science::Medicine
Human Microbiome
Inflammation
Tong, Louis
Constancias, Florentin
Hou, Aihua
Chua, Song Lin
Drautz-Moses, Daniela Isabel
Schuster, Stephan Christoph
Yang, Liang
Williams, Rohan B. H.
Kjelleberg, Staffan
Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
description The ocular surface microbiome has implications for ocular surface inflammation and immunology. Previous shotgun metagenomics analyses were performed in China, showing results that differed according to environment and age. Patients with Sjogren's syndrome were reported to have altered conjunctival microbiome, but such studies have not been done in milder dry eye. The aim of this study is to describe the conjunctival microbiome in people with mild dry eye in Singapore. Samples were collected from 14 participants with mild dry eye and 10 age-matched comparison participants recruited from Singapore National Eye Centre (SNEC) clinics. Shotgun metagenomic sequencing analysis was employed to evaluate the conjunctival microbiome composition. Proteobacteria formed the predominant phylum in the conjunctiva. As in a study from a coastal city in China, Achromobacter spp. was numerically most abundant. Compared to age-matched controls, the conjunctival microbial composition in mild dry eye was similar. Several microorganisms, including Streptococcus spp. increased in representation with age, and the abundance of Staphylococcus correlated with Schirmer readings. In addition, when cultured corneal epithelial cells were exposed to three strains of Achromobacter xylosoxidans, cytokines such as TNF-α and IL-6 were upregulated in the cell lysates and supernatants. Ourresults suggest that age is an important factor that affects composition of the conjunctival microbiome, and relative abundance of specific microorganism may vary according to the environment of the human host.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Tong, Louis
Constancias, Florentin
Hou, Aihua
Chua, Song Lin
Drautz-Moses, Daniela Isabel
Schuster, Stephan Christoph
Yang, Liang
Williams, Rohan B. H.
Kjelleberg, Staffan
format Article
author Tong, Louis
Constancias, Florentin
Hou, Aihua
Chua, Song Lin
Drautz-Moses, Daniela Isabel
Schuster, Stephan Christoph
Yang, Liang
Williams, Rohan B. H.
Kjelleberg, Staffan
author_sort Tong, Louis
title Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
title_short Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
title_full Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
title_fullStr Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
title_full_unstemmed Shotgun metagenomic sequencing analysis of ocular surface microbiome in Singapore residents with mild dry eye
title_sort shotgun metagenomic sequencing analysis of ocular surface microbiome in singapore residents with mild dry eye
publishDate 2023
url https://hdl.handle.net/10356/164505
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