Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synt...
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sg-ntu-dr.10356-1645152023-06-21T08:06:07Z Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers Li, Di Xiong, Qirong Liu, Wylie Liang, Li Duan, Hongwei School of Chemistry, Chemical Engineering and Biotechnology Science::Chemistry Nanozyme Magnetic Nanomixer Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synthesized by depositing a Fe2+-doped polydopamine coating on the surface of well-aligned magnetic nanoparticles to form a rigid chain-like nanostructure. Polydopamine coating of the nanozymatic MNE allows for efficient immobilization of natural enzymes such as glucose oxidase, cholesterol oxidase or urate oxidase to produce a series of enzymes-immobilized MNE (MNE@enzymes) with intrinsic multienzyme cascade properties. These MNE@enzymes show synchronously rotating capability in spinning magnetic fields, which leads to an 80∼100% improvement in their overall catalytic efficiencies. In the on-chip detection of small molecular metabolites (i.e., glucose, cholesterol, and uric acid), the rotating MNE@enzymes lead to detection sensitivities 2.1∼4.3 times higher than those of the static ones. Importantly, the consistent performance of the rotating MNE@enzymes offers the possibility of integrating the detection of glucose, free cholesterol and uric acid into a single multiplexing microchip assay with smartphone readout, affording an improved sensitivity, good selectivity and reliability. The designed enzymes-loaded MNEs holds great promise in developing rapid and ultrasensitive measurements of diverse targets of healthcare concerns using portable devices. Ministry of Education (MOE) Submitted/Accepted version This work was financially supported by Ministry of Education, Singapore (MOE2018-T2-2-128). 2023-01-30T06:17:40Z 2023-01-30T06:17:40Z 2023 Journal Article Li, D., Xiong, Q., Liu, W., Liang, L. & Duan, H. (2023). Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers. Biosensors and Bioelectronics, 219, 114795-. https://dx.doi.org/10.1016/j.bios.2022.114795 0956-5663 https://hdl.handle.net/10356/164515 10.1016/j.bios.2022.114795 36272348 2-s2.0-85140142831 219 114795 en MOE2018-T2-2-128 Biosensors and Bioelectronics © 2022 Elsevier B.V. A. All rights reserved. This paper was published in Biosensors and Bioelectronics and is made available with permission of Elsevier B.V. application/pdf application/pdf |
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Science::Chemistry Nanozyme Magnetic Nanomixer Li, Di Xiong, Qirong Liu, Wylie Liang, Li Duan, Hongwei Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
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Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synthesized by depositing a Fe2+-doped polydopamine coating on the surface of well-aligned magnetic nanoparticles to form a rigid chain-like nanostructure. Polydopamine coating of the nanozymatic MNE allows for efficient immobilization of natural enzymes such as glucose oxidase, cholesterol oxidase or urate oxidase to produce a series of enzymes-immobilized MNE (MNE@enzymes) with intrinsic multienzyme cascade properties. These MNE@enzymes show synchronously rotating capability in spinning magnetic fields, which leads to an 80∼100% improvement in their overall catalytic efficiencies. In the on-chip detection of small molecular metabolites (i.e., glucose, cholesterol, and uric acid), the rotating MNE@enzymes lead to detection sensitivities 2.1∼4.3 times higher than those of the static ones. Importantly, the consistent performance of the rotating MNE@enzymes offers the possibility of integrating the detection of glucose, free cholesterol and uric acid into a single multiplexing microchip assay with smartphone readout, affording an improved sensitivity, good selectivity and reliability. The designed enzymes-loaded MNEs holds great promise in developing rapid and ultrasensitive measurements of diverse targets of healthcare concerns using portable devices. |
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School of Chemistry, Chemical Engineering and Biotechnology |
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School of Chemistry, Chemical Engineering and Biotechnology Li, Di Xiong, Qirong Liu, Wylie Liang, Li Duan, Hongwei |
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Article |
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Li, Di Xiong, Qirong Liu, Wylie Liang, Li Duan, Hongwei |
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Li, Di |
title |
Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
title_short |
Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
title_full |
Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
title_fullStr |
Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
title_full_unstemmed |
Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
title_sort |
nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers |
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2023 |
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https://hdl.handle.net/10356/164515 |
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1772829004676464640 |