Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers

Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synt...

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Main Authors: Li, Di, Xiong, Qirong, Liu, Wylie, Liang, Li, Duan, Hongwei
Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164515
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1645152023-06-21T08:06:07Z Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers Li, Di Xiong, Qirong Liu, Wylie Liang, Li Duan, Hongwei School of Chemistry, Chemical Engineering and Biotechnology Science::Chemistry Nanozyme Magnetic Nanomixer Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synthesized by depositing a Fe2+-doped polydopamine coating on the surface of well-aligned magnetic nanoparticles to form a rigid chain-like nanostructure. Polydopamine coating of the nanozymatic MNE allows for efficient immobilization of natural enzymes such as glucose oxidase, cholesterol oxidase or urate oxidase to produce a series of enzymes-immobilized MNE (MNE@enzymes) with intrinsic multienzyme cascade properties. These MNE@enzymes show synchronously rotating capability in spinning magnetic fields, which leads to an 80∼100% improvement in their overall catalytic efficiencies. In the on-chip detection of small molecular metabolites (i.e., glucose, cholesterol, and uric acid), the rotating MNE@enzymes lead to detection sensitivities 2.1∼4.3 times higher than those of the static ones. Importantly, the consistent performance of the rotating MNE@enzymes offers the possibility of integrating the detection of glucose, free cholesterol and uric acid into a single multiplexing microchip assay with smartphone readout, affording an improved sensitivity, good selectivity and reliability. The designed enzymes-loaded MNEs holds great promise in developing rapid and ultrasensitive measurements of diverse targets of healthcare concerns using portable devices. Ministry of Education (MOE) Submitted/Accepted version This work was financially supported by Ministry of Education, Singapore (MOE2018-T2-2-128). 2023-01-30T06:17:40Z 2023-01-30T06:17:40Z 2023 Journal Article Li, D., Xiong, Q., Liu, W., Liang, L. & Duan, H. (2023). Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers. Biosensors and Bioelectronics, 219, 114795-. https://dx.doi.org/10.1016/j.bios.2022.114795 0956-5663 https://hdl.handle.net/10356/164515 10.1016/j.bios.2022.114795 36272348 2-s2.0-85140142831 219 114795 en MOE2018-T2-2-128 Biosensors and Bioelectronics © 2022 Elsevier B.V. A. All rights reserved. This paper was published in Biosensors and Bioelectronics and is made available with permission of Elsevier B.V. application/pdf application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Chemistry
Nanozyme
Magnetic Nanomixer
spellingShingle Science::Chemistry
Nanozyme
Magnetic Nanomixer
Li, Di
Xiong, Qirong
Liu, Wylie
Liang, Li
Duan, Hongwei
Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
description Nanozymes with enzyme-mimicking catalytic activity and unique functions have stimulated increasing interest in the biosensing field. Herein, we report a magnetic nanozyme (MNE) with integrated superior peroxidase-like activity and efficient mixing ability. This nanozymatic magnetic nanomixer is synthesized by depositing a Fe2+-doped polydopamine coating on the surface of well-aligned magnetic nanoparticles to form a rigid chain-like nanostructure. Polydopamine coating of the nanozymatic MNE allows for efficient immobilization of natural enzymes such as glucose oxidase, cholesterol oxidase or urate oxidase to produce a series of enzymes-immobilized MNE (MNE@enzymes) with intrinsic multienzyme cascade properties. These MNE@enzymes show synchronously rotating capability in spinning magnetic fields, which leads to an 80∼100% improvement in their overall catalytic efficiencies. In the on-chip detection of small molecular metabolites (i.e., glucose, cholesterol, and uric acid), the rotating MNE@enzymes lead to detection sensitivities 2.1∼4.3 times higher than those of the static ones. Importantly, the consistent performance of the rotating MNE@enzymes offers the possibility of integrating the detection of glucose, free cholesterol and uric acid into a single multiplexing microchip assay with smartphone readout, affording an improved sensitivity, good selectivity and reliability. The designed enzymes-loaded MNEs holds great promise in developing rapid and ultrasensitive measurements of diverse targets of healthcare concerns using portable devices.
author2 School of Chemistry, Chemical Engineering and Biotechnology
author_facet School of Chemistry, Chemical Engineering and Biotechnology
Li, Di
Xiong, Qirong
Liu, Wylie
Liang, Li
Duan, Hongwei
format Article
author Li, Di
Xiong, Qirong
Liu, Wylie
Liang, Li
Duan, Hongwei
author_sort Li, Di
title Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
title_short Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
title_full Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
title_fullStr Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
title_full_unstemmed Nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
title_sort nanozymatic magnetic nanomixers for enzyme immobilization and multiplexed detection of metabolic disease biomarkers
publishDate 2023
url https://hdl.handle.net/10356/164515
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