Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen

Microalgae used for CO2 biofixation can effectively relieve CO2 emissions and produce high-value biomass to achieve "waste-to-treasure" bioconversion. However, the low CO2 fixation efficiency and the restricted application of biomass are currently bottlenecks, limiting the economic viabili...

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Main Authors: Liu, Yu, Wei, Dong, Chen, Weining
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/164773
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spelling sg-ntu-dr.10356-1647732023-12-29T06:54:14Z Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen Liu, Yu Wei, Dong Chen, Weining School of Chemical and Biomedical Engineering Engineering::Bioengineering Oleaginous Microalga Coccomyxa Subellipsoidea Microalgae used for CO2 biofixation can effectively relieve CO2 emissions and produce high-value biomass to achieve "waste-to-treasure" bioconversion. However, the low CO2 fixation efficiency and the restricted application of biomass are currently bottlenecks, limiting the economic viability of CO2 biofixation by microalgae. To achieve high-efficient CO2 fixation and high-protein biomass production, the oleaginous microalga Coccomyxa subellipsoidea (C. subellipsoidea) was cultivated autotrophically through optimizing inorganic carbon and nitrogen supply. 0.42 g L-1 NaHCO3 supplemented with 2% CO2 as a hybrid carbon source resulted in high biomass concentration (3.89 g L-1) and productivity (318.33) with CO2 fixation rate 544.21 mg L-1 d-1 in shake flasks. Then, used in a 5-L photo-fermenter, the maximal protein content (60.93% DW) in batch 1, and the highest CO2 fixation rate (1043.95 mg L-1 d-1) with protein content (58.48% DW) in batch 2 of repeated fed-batch cultures were achieved under 2.5 g L-1 nitrate. The relative expression of key genes involved in photosynthesis, glycolysis, and protein synthesis showed significant upregulation. This study developed a promising approach for enhancing carbon allocation to protein synthesis in oleaginous microalga, facilitating the bioconversion of the fixed carbon into algal protein instead of oil in green manufacturing. Published version This work was supported by Guangdong Basic and Applied Basic Research Foundation (2019B1515120002). This work was partly supported by the 111 Project (B17018). 2023-02-14T01:40:50Z 2023-02-14T01:40:50Z 2022 Journal Article Liu, Y., Wei, D. & Chen, W. (2022). Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen. Frontiers in Bioengineering and Biotechnology, 10, 921024-. https://dx.doi.org/10.3389/fbioe.2022.921024 2296-4185 https://hdl.handle.net/10356/164773 10.3389/fbioe.2022.921024 35733523 2-s2.0-85133332398 10 921024 en Frontiers in Bioengineering and Biotechnology © 2022 Liu, Wei and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Bioengineering
Oleaginous Microalga
Coccomyxa Subellipsoidea
spellingShingle Engineering::Bioengineering
Oleaginous Microalga
Coccomyxa Subellipsoidea
Liu, Yu
Wei, Dong
Chen, Weining
Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
description Microalgae used for CO2 biofixation can effectively relieve CO2 emissions and produce high-value biomass to achieve "waste-to-treasure" bioconversion. However, the low CO2 fixation efficiency and the restricted application of biomass are currently bottlenecks, limiting the economic viability of CO2 biofixation by microalgae. To achieve high-efficient CO2 fixation and high-protein biomass production, the oleaginous microalga Coccomyxa subellipsoidea (C. subellipsoidea) was cultivated autotrophically through optimizing inorganic carbon and nitrogen supply. 0.42 g L-1 NaHCO3 supplemented with 2% CO2 as a hybrid carbon source resulted in high biomass concentration (3.89 g L-1) and productivity (318.33) with CO2 fixation rate 544.21 mg L-1 d-1 in shake flasks. Then, used in a 5-L photo-fermenter, the maximal protein content (60.93% DW) in batch 1, and the highest CO2 fixation rate (1043.95 mg L-1 d-1) with protein content (58.48% DW) in batch 2 of repeated fed-batch cultures were achieved under 2.5 g L-1 nitrate. The relative expression of key genes involved in photosynthesis, glycolysis, and protein synthesis showed significant upregulation. This study developed a promising approach for enhancing carbon allocation to protein synthesis in oleaginous microalga, facilitating the bioconversion of the fixed carbon into algal protein instead of oil in green manufacturing.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Liu, Yu
Wei, Dong
Chen, Weining
format Article
author Liu, Yu
Wei, Dong
Chen, Weining
author_sort Liu, Yu
title Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
title_short Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
title_full Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
title_fullStr Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
title_full_unstemmed Oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for CO₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
title_sort oleaginous microalga coccomyxa subellipsoidea as a highly effective cell factory for co₂ fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen
publishDate 2023
url https://hdl.handle.net/10356/164773
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