Controlled 3D ordered protein/conducting polymer nanocomposite film and its direct electrochemistry.

• Main Author: Sui, Ning.
• Other Authors: Li Changming
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Engineering::Chemical engineering::Polymers and polymer manufacture
• Online Access: http://hdl.handle.net/10356/16509
• Institution: Nanyang Technological University

In the present work, three-dimensionally (3D) ordered macroporous glucose oxidase/conducting polymer nanocomposite film was synthesized by using the inverted colloidal polystyrene crystal template technique and in-situ copolymerization method. The structure of the pore can be precisely controlled by the calibrated relation between film thickness and deposition time. The in-situ copolymerization method allows proteins distributing uniformly inside conducting polymer film with high loading amount, which was confirmed by confocal microscopy. The 3D macroporous conducting polymer film also provides a good microenvironment for retaining the nature structure of the entrapped enzymes, as verified by attenuated total reflection Fourier-transform infrared spectroscopy. More importantly, direct electron transfer of proteins in the 3D nanocomposite film was observed, which provides the potential applications in third-generation biosensor.