Changes in mechanical properties of vesicles by mucin in aqueous solution

The mechanical properties of vesicles were investigated as they were prepared, according to the ratio of mucin to dipalmitoylphosphatidylcholine (DPPC), using an atomic force microscope (AFM). After the confirmation of the vesicle adsorption on a mica surface, an AFM-tip deflection, caused by the in...

Full description

Saved in:
Bibliographic Details
Main Authors: Lee, Gaeul, Hadinoto, Kunn, Park, Jin-Won
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2023
Subjects:
Online Access:https://hdl.handle.net/10356/165143
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-165143
record_format dspace
spelling sg-ntu-dr.10356-1651432023-12-29T06:50:33Z Changes in mechanical properties of vesicles by mucin in aqueous solution Lee, Gaeul Hadinoto, Kunn Park, Jin-Won School of Chemical and Biomedical Engineering Engineering::Chemical engineering Mucin Mechanical Properties The mechanical properties of vesicles were investigated as they were prepared, according to the ratio of mucin to dipalmitoylphosphatidylcholine (DPPC), using an atomic force microscope (AFM). After the confirmation of the vesicle adsorption on a mica surface, an AFM-tip deflection, caused by the interaction between the tip and the vesicle, was measured. The deflection showed that the tip broke through into the vesicle twice. Each break meant a tip-penetration into the upper and lower portion of the vesicle. Only the first penetration allowed the Hertzian model available to estimate the vesicle mechanical moduli. Two moduli reduced as the ratio of mucin to DPPC increased to 0.5, but the moduli were little changed above the 0.5 ratio. These results seem to be a platform for the effect of the mucin on the plasma-membrane anchoring and cellular signaling. Published version 2023-03-15T00:32:06Z 2023-03-15T00:32:06Z 2022 Journal Article Lee, G., Hadinoto, K. & Park, J. (2022). Changes in mechanical properties of vesicles by mucin in aqueous solution. Nanomaterials (Basel, Switzerland), 12(20), 12203683-. https://dx.doi.org/10.3390/nano12203683 2079-4991 https://hdl.handle.net/10356/165143 10.3390/nano12203683 36296873 2-s2.0-85140729219 20 12 12203683 en Nanomaterials (Basel, Switzerland) © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Chemical engineering
Mucin
Mechanical Properties
spellingShingle Engineering::Chemical engineering
Mucin
Mechanical Properties
Lee, Gaeul
Hadinoto, Kunn
Park, Jin-Won
Changes in mechanical properties of vesicles by mucin in aqueous solution
description The mechanical properties of vesicles were investigated as they were prepared, according to the ratio of mucin to dipalmitoylphosphatidylcholine (DPPC), using an atomic force microscope (AFM). After the confirmation of the vesicle adsorption on a mica surface, an AFM-tip deflection, caused by the interaction between the tip and the vesicle, was measured. The deflection showed that the tip broke through into the vesicle twice. Each break meant a tip-penetration into the upper and lower portion of the vesicle. Only the first penetration allowed the Hertzian model available to estimate the vesicle mechanical moduli. Two moduli reduced as the ratio of mucin to DPPC increased to 0.5, but the moduli were little changed above the 0.5 ratio. These results seem to be a platform for the effect of the mucin on the plasma-membrane anchoring and cellular signaling.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Lee, Gaeul
Hadinoto, Kunn
Park, Jin-Won
format Article
author Lee, Gaeul
Hadinoto, Kunn
Park, Jin-Won
author_sort Lee, Gaeul
title Changes in mechanical properties of vesicles by mucin in aqueous solution
title_short Changes in mechanical properties of vesicles by mucin in aqueous solution
title_full Changes in mechanical properties of vesicles by mucin in aqueous solution
title_fullStr Changes in mechanical properties of vesicles by mucin in aqueous solution
title_full_unstemmed Changes in mechanical properties of vesicles by mucin in aqueous solution
title_sort changes in mechanical properties of vesicles by mucin in aqueous solution
publishDate 2023
url https://hdl.handle.net/10356/165143
_version_ 1787136682620878848