Investigation of cellular changes on blood cells relevant for the destabilization of blood flow

Increased red blood cell adherence to endothelial cells has been found in diseases including sickle cell anemia, β-thalassaemia, diabetes mellitus, and malaria, in which red blood cells undergo various physicochemical changes on the membrane, leading to altered adhesion to endothelial cells. The cu...

Full description

Saved in:
Bibliographic Details
Main Author: Stephanie.
Other Authors: Bjoern Holger Neu
Format: Final Year Project
Language:English
Published: 2009
Subjects:
Online Access:http://hdl.handle.net/10356/16517
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-16517
record_format dspace
spelling sg-ntu-dr.10356-165172023-03-03T15:41:21Z Investigation of cellular changes on blood cells relevant for the destabilization of blood flow Stephanie. Bjoern Holger Neu School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biochemical engineering Increased red blood cell adherence to endothelial cells has been found in diseases including sickle cell anemia, β-thalassaemia, diabetes mellitus, and malaria, in which red blood cells undergo various physicochemical changes on the membrane, leading to altered adhesion to endothelial cells. The current study mimicked these changes by using different enzyme treatments (neuraminidase, alpha-chymotrypsin, trypsin, and pronase) and investigated them by measuring zeta potential (surface charge) and deformability of treated cells as well as conducting gel electrophoresis. The adhesion was analyzed on a parallel plate flow chamber system. Zeta potential was increased after enzymatic treatments, with neuraminidase found to be the most effective in removing surface charge. Deformability was reduced in all enzyme-treated cells. Membrane composition changes were observed in all enzymes except neuraminidase. The presence of dextran, a macromolecule inducing depletion interaction used to mimic the impact of plasma, was found to significantly enhance the adhesion of both normal and enzyme-treated cells. In conclusion, depletion interaction is a significant determinant in red blood cell – endothelial cell adhesion, electrostatic repulsion is the key nonspecific force governing cell – cell interaction, and deformability reduction alters cell rheological properties which in turn reduce red blood cell – endothelial cell adhesion. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-27T01:22:38Z 2009-05-27T01:22:38Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16517 en Nanyang Technological University 65 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biochemical engineering
spellingShingle DRNTU::Engineering::Chemical engineering::Biochemical engineering
Stephanie.
Investigation of cellular changes on blood cells relevant for the destabilization of blood flow
description Increased red blood cell adherence to endothelial cells has been found in diseases including sickle cell anemia, β-thalassaemia, diabetes mellitus, and malaria, in which red blood cells undergo various physicochemical changes on the membrane, leading to altered adhesion to endothelial cells. The current study mimicked these changes by using different enzyme treatments (neuraminidase, alpha-chymotrypsin, trypsin, and pronase) and investigated them by measuring zeta potential (surface charge) and deformability of treated cells as well as conducting gel electrophoresis. The adhesion was analyzed on a parallel plate flow chamber system. Zeta potential was increased after enzymatic treatments, with neuraminidase found to be the most effective in removing surface charge. Deformability was reduced in all enzyme-treated cells. Membrane composition changes were observed in all enzymes except neuraminidase. The presence of dextran, a macromolecule inducing depletion interaction used to mimic the impact of plasma, was found to significantly enhance the adhesion of both normal and enzyme-treated cells. In conclusion, depletion interaction is a significant determinant in red blood cell – endothelial cell adhesion, electrostatic repulsion is the key nonspecific force governing cell – cell interaction, and deformability reduction alters cell rheological properties which in turn reduce red blood cell – endothelial cell adhesion.
author2 Bjoern Holger Neu
author_facet Bjoern Holger Neu
Stephanie.
format Final Year Project
author Stephanie.
author_sort Stephanie.
title Investigation of cellular changes on blood cells relevant for the destabilization of blood flow
title_short Investigation of cellular changes on blood cells relevant for the destabilization of blood flow
title_full Investigation of cellular changes on blood cells relevant for the destabilization of blood flow
title_fullStr Investigation of cellular changes on blood cells relevant for the destabilization of blood flow
title_full_unstemmed Investigation of cellular changes on blood cells relevant for the destabilization of blood flow
title_sort investigation of cellular changes on blood cells relevant for the destabilization of blood flow
publishDate 2009
url http://hdl.handle.net/10356/16517
_version_ 1759858299218427904