Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach

Chemotherapy is a common treatment for breast cancer and Doxorubicin is a common chemotherapeutic drug used for this purpose. However drug resistance is a great limitation to the effectiveness of chemotherapy treatment. It has been discovered that patients with single nucleotide polymorphism (SNP) i...

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Main Author: Cheah, Chee Liang.
Other Authors: Chen Wei Ning, William
Format: Final Year Project
Language:English
Published: 2009
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Online Access:http://hdl.handle.net/10356/16544
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-165442023-03-03T15:37:32Z Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach Cheah, Chee Liang. Chen Wei Ning, William School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology Chemotherapy is a common treatment for breast cancer and Doxorubicin is a common chemotherapeutic drug used for this purpose. However drug resistance is a great limitation to the effectiveness of chemotherapy treatment. It has been discovered that patients with single nucleotide polymorphism (SNP) in Murine Double Minute 2 (MDM-2) gene promoter have higher resistance to chemotherapeutic treatment with Doxorubicin. In this project, iTRAQ-coupled LC-MS/MS approach was used to study the protein profile of breast cancer patients undergoing Doxorubicin treatment. Protein profile of 23 patient plasma samples with SNP309 (both homogenous and heterogeneous) in MDM-2 Gene was compared with the protein profile of control sample made up by 7 patient plasma samples with wild type (WT) MDM-2 Gene. The online 2D LC-MS/MS system used involved a two dimensional application of a strong cation exchange column followed by a reverse-phased column, coupled to a tandem mass spectrometer. This approach also involved the use of four isobaric peptide derivatization reagents (iTRAQ reagents) to yield informative spectra for peptide identification and quantitation. The experimental results from this project showed that 2 proteins, Fibrinogen alpha chain precursor and Fibrinogen gamma chain precursor, have higher expression levels in patients with SNP309 in MDM-2 gene. This indicates that Fibrinogen may play an important role in MDM-2 mediated Doxorubicin resistance. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-05-27T02:42:49Z 2009-05-27T02:42:49Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/16544 en Nanyang Technological University 57 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
Cheah, Chee Liang.
Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach
description Chemotherapy is a common treatment for breast cancer and Doxorubicin is a common chemotherapeutic drug used for this purpose. However drug resistance is a great limitation to the effectiveness of chemotherapy treatment. It has been discovered that patients with single nucleotide polymorphism (SNP) in Murine Double Minute 2 (MDM-2) gene promoter have higher resistance to chemotherapeutic treatment with Doxorubicin. In this project, iTRAQ-coupled LC-MS/MS approach was used to study the protein profile of breast cancer patients undergoing Doxorubicin treatment. Protein profile of 23 patient plasma samples with SNP309 (both homogenous and heterogeneous) in MDM-2 Gene was compared with the protein profile of control sample made up by 7 patient plasma samples with wild type (WT) MDM-2 Gene. The online 2D LC-MS/MS system used involved a two dimensional application of a strong cation exchange column followed by a reverse-phased column, coupled to a tandem mass spectrometer. This approach also involved the use of four isobaric peptide derivatization reagents (iTRAQ reagents) to yield informative spectra for peptide identification and quantitation. The experimental results from this project showed that 2 proteins, Fibrinogen alpha chain precursor and Fibrinogen gamma chain precursor, have higher expression levels in patients with SNP309 in MDM-2 gene. This indicates that Fibrinogen may play an important role in MDM-2 mediated Doxorubicin resistance.
author2 Chen Wei Ning, William
author_facet Chen Wei Ning, William
Cheah, Chee Liang.
format Final Year Project
author Cheah, Chee Liang.
author_sort Cheah, Chee Liang.
title Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach
title_short Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach
title_full Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach
title_fullStr Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach
title_full_unstemmed Protein profile in breast cancer patients undergoing doxorubicin treatment : an iTRAQ-coupled LC-MS/MS approach
title_sort protein profile in breast cancer patients undergoing doxorubicin treatment : an itraq-coupled lc-ms/ms approach
publishDate 2009
url http://hdl.handle.net/10356/16544
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