Effects of Neu3 on the dynamics of flotillin 1 dependent endocytosis
Hog cells were transfected with Neu3-EGFP (Enhanced green fluorescent protein), flotillin 1-EGFP or Neu3-HA and flotillin 1-EGFP to test the effect that Neu3 has on the dynamics of flotillin 1 dependent endocytosis. Cells that were transfected with Neu3-HA and flotillin 1-EGFP showed lower lateral a...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/16574 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Hog cells were transfected with Neu3-EGFP (Enhanced green fluorescent protein), flotillin 1-EGFP or Neu3-HA and flotillin 1-EGFP to test the effect that Neu3 has on the dynamics of flotillin 1 dependent endocytosis. Cells that were transfected with Neu3-HA and flotillin 1-EGFP showed lower lateral average speeds and longer dwell time, implying overall lower average speed compared to those transfected with flotillin 1 only. Also, they showed lower vesicular movement in general as evident from their lower CDLong values over time compared to flotillin 1 transfected cells. They also have a different movement as evident from the different CDLong, CDArea, CDshort and aspect ratio. The average budding rate in cells transfected with Neu3-HA and flotillin 1-EGFP is also slower than cells transfected with flotillin 1 only. |
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