Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays

Pesticides are an indispensable part of agriculture in the modern day to enhance crop yield and pest control. Despite increasing recognition of the health and environmental hazards derived from these complex chemical mixtures, approval of agrochemical products is mostly dependent on the toxicity of...

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Main Author: Tan, Roy Zi Hao
Other Authors: Federico Lauro
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
Subjects:
Online Access:https://hdl.handle.net/10356/165743
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1657432023-04-10T15:32:22Z Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays Tan, Roy Zi Hao Federico Lauro Shane Allen Snyder Asian School of the Environment Nanyang Environment and Water Research Institute FLauro@ntu.edu.sg, ssnyder@ntu.edu.sg Engineering::Environmental engineering::Environmental pollution Pesticides are an indispensable part of agriculture in the modern day to enhance crop yield and pest control. Despite increasing recognition of the health and environmental hazards derived from these complex chemical mixtures, approval of agrochemical products is mostly dependent on the toxicity of individual components, disregarding potential combined effects. The objective of the study was to assess the toxicity of common commercial agropesticides using both cell-line and zebrafish embryonic assays as model organisms, as well as briefly evaluate environmental waters in agriculture-dominated sites using Chiang Mai, Northern Thailand as a case study. The results showed that the pesticides tested exhibited acute lethality and sublethal effects on the model organisms tested, albeit some to a lesser extent than previously reported findings on single active compounds. Our findings also revealed challenges in using a single broad-spectrum solid-phase extraction method to retain pesticide analytes due to their chemical complexity. Nonetheless, the study presents a holistic workflow in assessing the ecotoxicity of environmental water samples. The water samples from Chiang Mai significantly induced endocrine responses in both assays, with transcriptome data indicating deregulation in major developmental gene expression pathways after a 96h exposure. These findings confirm the presence of contaminants in the aquatic environment of agriculture-dominated regions, which can potentially alter physiological development in exposed organisms and decrease overall fitness. We recommend that all pesticide formulations be fully considered in authorization processes and structured environmental monitoring schemes be developed to mitigate the negative impacts of agrochemicals on both human health and ecosystem stability. Bachelor of Science in Environmental Earth Systems Science 2023-04-10T02:00:35Z 2023-04-10T02:00:35Z 2023 Final Year Project (FYP) Tan, R. Z. H. (2023). Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/165743 https://hdl.handle.net/10356/165743 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering::Environmental pollution
spellingShingle Engineering::Environmental engineering::Environmental pollution
Tan, Roy Zi Hao
Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
description Pesticides are an indispensable part of agriculture in the modern day to enhance crop yield and pest control. Despite increasing recognition of the health and environmental hazards derived from these complex chemical mixtures, approval of agrochemical products is mostly dependent on the toxicity of individual components, disregarding potential combined effects. The objective of the study was to assess the toxicity of common commercial agropesticides using both cell-line and zebrafish embryonic assays as model organisms, as well as briefly evaluate environmental waters in agriculture-dominated sites using Chiang Mai, Northern Thailand as a case study. The results showed that the pesticides tested exhibited acute lethality and sublethal effects on the model organisms tested, albeit some to a lesser extent than previously reported findings on single active compounds. Our findings also revealed challenges in using a single broad-spectrum solid-phase extraction method to retain pesticide analytes due to their chemical complexity. Nonetheless, the study presents a holistic workflow in assessing the ecotoxicity of environmental water samples. The water samples from Chiang Mai significantly induced endocrine responses in both assays, with transcriptome data indicating deregulation in major developmental gene expression pathways after a 96h exposure. These findings confirm the presence of contaminants in the aquatic environment of agriculture-dominated regions, which can potentially alter physiological development in exposed organisms and decrease overall fitness. We recommend that all pesticide formulations be fully considered in authorization processes and structured environmental monitoring schemes be developed to mitigate the negative impacts of agrochemicals on both human health and ecosystem stability.
author2 Federico Lauro
author_facet Federico Lauro
Tan, Roy Zi Hao
format Final Year Project
author Tan, Roy Zi Hao
author_sort Tan, Roy Zi Hao
title Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
title_short Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
title_full Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
title_fullStr Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
title_full_unstemmed Evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
title_sort evaluating the ecotoxicity of agropesticides and environmental water contaminants using cell-line and zebrafish embryonic assays
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/165743
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