Application of MCG-PTCC system on neo-cartilage regeneration

• Main Author: Jiang, Yuwei
• Other Authors: Wang Dongan
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnology
• Online Access: http://hdl.handle.net/10356/16579
• Institution: Nanyang Technological University

This project aims to apply the advance fabricated micro-cavities gel (MCG) construct and the innovative phase transfer cell culture (PTCC) strategy to cartilage tissue engineering to realize the self-formation of the bone fide neo-cartilage tissue. The phenomenon called edge flourish (EF) is the underlying principle for successfully creating the MCG-PTCC system. The efficiency of the MCG-PTCC system on the cartilage regeneration is evaluated in terms of chondrocytes viability, development of the neo-cartilage tissue islets, related gene expression level, extracellular matrix (ECM) proteins production level. At the same time, the MCG-PTCC system is compared with the traditional therapeutic cell encapsulated hydrogel construct to prove its superiority.