Probiotic vesicles as supplement for inflammatory bowel disease treatment

• Main Author: Pun, Song Yi
• Other Authors: Czarny Bertrand Marcel Stanislas
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2023
• Subjects: Engineering::Materials::Biomaterials
• Online Access: https://hdl.handle.net/10356/165801
• Institution: Nanyang Technological University

Extracellular Vesicles (EVs) are nanosized particles secreted by different cell types that play a crucial role in transmitting signals and modulating physiological processes in the host. As an emerging area of research, particularly in biomedicine, EVs hold great promise for a wide range of applications. Lactobacillus rhamnosus GG (LGG), a probiotic strain that has been shown to offer a variety of health benefits to host when administered. However, the potential of LGG EVs in modulating host-microbe interactions remains largely unexplored. Therefore, exploring the role of LGG EVs in host-microbe interactions could prove to be a new avenue of research in the field of biomedicine. By studying the effects of LGG EVs on the host cells and their potential in treating inflammation, we may be able to unlock new therapeutic strategies and advance our understanding of the role of EVs in regulating biological processes. This study aimed to develop protocol to isolate both Natural EVs (NEVs) and Mimetic EVs (MEVs) from the LGG bacteria culture. We studied LGG EVs produced with different characterization methods to optimize production protocol for medicinal applications. These findings could have significant implications for the potential commercialization of LGG EVs in medicine and can help pave the way for future research in this field. In addition, we investigated the host-cell response between LGG EVs with mice cell lines using MTT assay and qPCR. Different concentrations of LGG EVs were used to treat RAW 264.7 macrophage to evaluate LGG EVs cytotoxicity and inflammatory response. Our results demonstrated that LGG EVs were able to promote cell proliferation at appropriate concentration. Moreover, we observed that LGG EVs can modulate cytokine production in RAW 264.7 macrophages to facilitate immune response. These findings suggest that LGG-derived EVs could be used as novel therapeutics in IBD and offer significant potential for improving human health.