Optimisation of the production of nanovesicles from phytopathogenic bacterial species
The use of bacteria derived nanovesicles (BDNs) for the development of medical treatments have been well documented in literature. However, the use of BDNs derived from phytopathogenic bacterial species for the treatment of plants has not yet been well studied. This project aimed to optimise a pr...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
Nanyang Technological University
2023
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| Online Access: | https://hdl.handle.net/10356/165955 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The use of bacteria derived nanovesicles (BDNs) for the development of medical treatments
have been well documented in literature. However, the use of BDNs derived from
phytopathogenic bacterial species for the treatment of plants has not yet been well studied.
This project aimed to optimise a production protocol of mimetic extracellular vesicles from
the bacterial species Pseudomonas Syringae using spin cups as an extrusion method, with the
hope that it may be scaled up to an industrial level. The yield of extracellular vesicles by
using a memetic production method was then compared against the vesicle yield that was
naturally produced by Pseudomonas Syringae. It was found that both methods are similar in
vesicle yields and that the use of spin cups to produce extracellular vesicles do not provide
much of an advantage over the method used to produce BDNs naturally. |
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