GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins

The Golgi complex is made up of cisternae stacks that are divided into multiple zones. Previous study from our lab showed that Golgi proteins partition to the interior or rim within the same cisterna, but the mechanisms for differential localization remains unknown. GPP130 and ST6GAL1 are type II tr...

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Main Author: Wong, Jing Xue
Other Authors: Lu Lei
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
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Online Access:https://hdl.handle.net/10356/166454
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spelling sg-ntu-dr.10356-1664542023-05-08T15:33:35Z GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins Wong, Jing Xue Lu Lei School of Biological Sciences LULEI@ntu.edu.sg Science::Biological sciences The Golgi complex is made up of cisternae stacks that are divided into multiple zones. Previous study from our lab showed that Golgi proteins partition to the interior or rim within the same cisterna, but the mechanisms for differential localization remains unknown. GPP130 and ST6GAL1 are type II transmembrane proteins that localize to the trans-Golgi. However, GPP130 is rim-localized, while ST6GAL1 is interior-localized. In this study, we attempt to find GPP130 domain that is responsible for intra-Golgi cisternal rim localization. To that end, we swapped domains between GPP130 and ST6GAL1 to make chimera proteins and performed super-resolution microscopy to study their cisternal localization. We demonstrated that the GPP130 stem region is necessary for the rim localization of the chimera proteins. Next, we fused GPP130 stem region with non-Golgi localized type II transmembrane proteins, TfR and TNF-α. We found that the GPP130 stem region was sufficient to localize TfR and TNF-α to the Golgi at the cisternal rim. Using Alphafold2, we found that the GPP130 stem region assembles a continuous and dimeric helical coiled-coil with the transmembrane domain. This could be a novel membrane curvature sensing module that brings GPP130 to the high curvature region of the Golgi cisternal rim. Bachelor of Science in Biological Sciences 2023-05-05T07:52:26Z 2023-05-05T07:52:26Z 2023 Final Year Project (FYP) Wong, J. X. (2023). GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/166454 https://hdl.handle.net/10356/166454 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Wong, Jing Xue
GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins
description The Golgi complex is made up of cisternae stacks that are divided into multiple zones. Previous study from our lab showed that Golgi proteins partition to the interior or rim within the same cisterna, but the mechanisms for differential localization remains unknown. GPP130 and ST6GAL1 are type II transmembrane proteins that localize to the trans-Golgi. However, GPP130 is rim-localized, while ST6GAL1 is interior-localized. In this study, we attempt to find GPP130 domain that is responsible for intra-Golgi cisternal rim localization. To that end, we swapped domains between GPP130 and ST6GAL1 to make chimera proteins and performed super-resolution microscopy to study their cisternal localization. We demonstrated that the GPP130 stem region is necessary for the rim localization of the chimera proteins. Next, we fused GPP130 stem region with non-Golgi localized type II transmembrane proteins, TfR and TNF-α. We found that the GPP130 stem region was sufficient to localize TfR and TNF-α to the Golgi at the cisternal rim. Using Alphafold2, we found that the GPP130 stem region assembles a continuous and dimeric helical coiled-coil with the transmembrane domain. This could be a novel membrane curvature sensing module that brings GPP130 to the high curvature region of the Golgi cisternal rim.
author2 Lu Lei
author_facet Lu Lei
Wong, Jing Xue
format Final Year Project
author Wong, Jing Xue
author_sort Wong, Jing Xue
title GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins
title_short GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins
title_full GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins
title_fullStr GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins
title_full_unstemmed GPP130 stem region is necessary and sufficient for intra-Golgi cisternal rim localization of type II transmembrane proteins
title_sort gpp130 stem region is necessary and sufficient for intra-golgi cisternal rim localization of type ii transmembrane proteins
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/166454
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