Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants

Given the interest in medicinal plants that have potential pain-relieving effects without the side effects of NSAIDS and opioids, secondary metabolite pathways in plants are being studied extensively. However, there are several valuable compounds for which the biosynthetic pathways are yet to be...

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Main Author: Menon, Devika
Other Authors: Marek Mutwil
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
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Online Access:https://hdl.handle.net/10356/167088
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1670882023-05-22T15:35:20Z Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants Menon, Devika Marek Mutwil School of Biological Sciences mutwil@ntu.edu.sg Science::Biological sciences Given the interest in medicinal plants that have potential pain-relieving effects without the side effects of NSAIDS and opioids, secondary metabolite pathways in plants are being studied extensively. However, there are several valuable compounds for which the biosynthetic pathways are yet to be understood. Hence, the study aimed to investigate the relevant secondary metabolite pathways in three species of medicinal plants which are known to have analgesic properties - P. granatum, A. paniculata, T. divaricata. In P. granatum the pathways studied were flavenoid biosynthesis pathways and Anthocyanin biosynthesis pathways. For both these pathways, key enzymes were identified after annotation using E2P2 and PathoLogic on PathwayTools. Pathways in A. paniculata were studied similarly, but were focused on the synthesis of andrographolide. The pathways that lead to the synthesis of an andrographolide precursor were identified to have high frequency scores and the enzymes involved were identified as well. Finally, RNA samples of T.divaricata from a dwarf and wild-type plant were extracted and transcriptomes from two organs of the dwarf plant were assembled towards the pathway analysis. Bachelor of Science in Biological Sciences 2023-05-21T12:49:03Z 2023-05-21T12:49:03Z 2023 Final Year Project (FYP) Menon, D. (2023). Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/167088 https://hdl.handle.net/10356/167088 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Menon, Devika
Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
description Given the interest in medicinal plants that have potential pain-relieving effects without the side effects of NSAIDS and opioids, secondary metabolite pathways in plants are being studied extensively. However, there are several valuable compounds for which the biosynthetic pathways are yet to be understood. Hence, the study aimed to investigate the relevant secondary metabolite pathways in three species of medicinal plants which are known to have analgesic properties - P. granatum, A. paniculata, T. divaricata. In P. granatum the pathways studied were flavenoid biosynthesis pathways and Anthocyanin biosynthesis pathways. For both these pathways, key enzymes were identified after annotation using E2P2 and PathoLogic on PathwayTools. Pathways in A. paniculata were studied similarly, but were focused on the synthesis of andrographolide. The pathways that lead to the synthesis of an andrographolide precursor were identified to have high frequency scores and the enzymes involved were identified as well. Finally, RNA samples of T.divaricata from a dwarf and wild-type plant were extracted and transcriptomes from two organs of the dwarf plant were assembled towards the pathway analysis.
author2 Marek Mutwil
author_facet Marek Mutwil
Menon, Devika
format Final Year Project
author Menon, Devika
author_sort Menon, Devika
title Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
title_short Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
title_full Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
title_fullStr Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
title_full_unstemmed Study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
title_sort study of secondary metabolite pathways that contribute to analgesic activity in three species of medicinal plants
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/167088
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