Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins

Nature has long been a source of inspiration for the design of advanced biomaterials with exceptional properties. One such inspiration is the Club Mineralization Protein-1, that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs. CMP-113, a truncated fusion prote...

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Main Author: Koh, Jing Han
Other Authors: Konstantin Pervushin
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2023
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Online Access:https://hdl.handle.net/10356/167170
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spelling sg-ntu-dr.10356-1671702023-05-29T15:33:08Z Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins Koh, Jing Han Konstantin Pervushin School of Biological Sciences KPervushin@ntu.edu.sg Science::Biological sciences Nature has long been a source of inspiration for the design of advanced biomaterials with exceptional properties. One such inspiration is the Club Mineralization Protein-1, that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs. CMP-113, a truncated fusion protein inspired by the CMP-1, was designed to incorporate liquid-liquid phase separation (LLPS)-promoting motifs to investigate how phase separation can potentially contribute to biomineralization. In the presence of various Hofmeister salts, coacervation of CMP-113 was assessed through brightfield microscopy, turbidity assays, dynamic light scattering (DLS) measurements and nuclear magnetic resonance (NMR) spectroscopy. Its resistance to 1,6-hexanediol treatment and re-entrant LLPS in presence of calcium chloride suggests that LLPS is not purely hydrophobic-driven but may potentially electrostatic interactions as well. Morphological observations of crystals formed in biomineralization experiments not only demonstrated CMP-113’s capability to form hydroxyapatite from solution, but its coacervation may also be imperative for accelerating crystal nucleation and growth. These findings were consistent with the wider literature on non-classical nucleation pathways of biomineralization. Future research on CMP-113 and its applications in biomaterials and materials science is anticipated to unveil new insights and open up exciting opportunities in the field of biomaterials research. Bachelor of Science in Biological Sciences and Psychology 2023-05-23T14:39:07Z 2023-05-23T14:39:07Z 2023 Final Year Project (FYP) Koh, J. H. (2023). Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/167170 https://hdl.handle.net/10356/167170 en MOE 2019-T3-1-012 application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Koh, Jing Han
Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
description Nature has long been a source of inspiration for the design of advanced biomaterials with exceptional properties. One such inspiration is the Club Mineralization Protein-1, that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs. CMP-113, a truncated fusion protein inspired by the CMP-1, was designed to incorporate liquid-liquid phase separation (LLPS)-promoting motifs to investigate how phase separation can potentially contribute to biomineralization. In the presence of various Hofmeister salts, coacervation of CMP-113 was assessed through brightfield microscopy, turbidity assays, dynamic light scattering (DLS) measurements and nuclear magnetic resonance (NMR) spectroscopy. Its resistance to 1,6-hexanediol treatment and re-entrant LLPS in presence of calcium chloride suggests that LLPS is not purely hydrophobic-driven but may potentially electrostatic interactions as well. Morphological observations of crystals formed in biomineralization experiments not only demonstrated CMP-113’s capability to form hydroxyapatite from solution, but its coacervation may also be imperative for accelerating crystal nucleation and growth. These findings were consistent with the wider literature on non-classical nucleation pathways of biomineralization. Future research on CMP-113 and its applications in biomaterials and materials science is anticipated to unveil new insights and open up exciting opportunities in the field of biomaterials research.
author2 Konstantin Pervushin
author_facet Konstantin Pervushin
Koh, Jing Han
format Final Year Project
author Koh, Jing Han
author_sort Koh, Jing Han
title Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
title_short Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
title_full Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
title_fullStr Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
title_full_unstemmed Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
title_sort investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
publisher Nanyang Technological University
publishDate 2023
url https://hdl.handle.net/10356/167170
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