Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins
Nature has long been a source of inspiration for the design of advanced biomaterials with exceptional properties. One such inspiration is the Club Mineralization Protein-1, that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs. CMP-113, a truncated fusion prote...
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sg-ntu-dr.10356-1671702023-05-29T15:33:08Z Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins Koh, Jing Han Konstantin Pervushin School of Biological Sciences KPervushin@ntu.edu.sg Science::Biological sciences Nature has long been a source of inspiration for the design of advanced biomaterials with exceptional properties. One such inspiration is the Club Mineralization Protein-1, that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs. CMP-113, a truncated fusion protein inspired by the CMP-1, was designed to incorporate liquid-liquid phase separation (LLPS)-promoting motifs to investigate how phase separation can potentially contribute to biomineralization. In the presence of various Hofmeister salts, coacervation of CMP-113 was assessed through brightfield microscopy, turbidity assays, dynamic light scattering (DLS) measurements and nuclear magnetic resonance (NMR) spectroscopy. Its resistance to 1,6-hexanediol treatment and re-entrant LLPS in presence of calcium chloride suggests that LLPS is not purely hydrophobic-driven but may potentially electrostatic interactions as well. Morphological observations of crystals formed in biomineralization experiments not only demonstrated CMP-113’s capability to form hydroxyapatite from solution, but its coacervation may also be imperative for accelerating crystal nucleation and growth. These findings were consistent with the wider literature on non-classical nucleation pathways of biomineralization. Future research on CMP-113 and its applications in biomaterials and materials science is anticipated to unveil new insights and open up exciting opportunities in the field of biomaterials research. Bachelor of Science in Biological Sciences and Psychology 2023-05-23T14:39:07Z 2023-05-23T14:39:07Z 2023 Final Year Project (FYP) Koh, J. H. (2023). Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/167170 https://hdl.handle.net/10356/167170 en MOE 2019-T3-1-012 application/pdf Nanyang Technological University |
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Science::Biological sciences Koh, Jing Han Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
description |
Nature has long been a source of inspiration for the design of advanced biomaterials
with exceptional properties. One such inspiration is the Club Mineralization Protein-1,
that plays a crucial role in the biomineralization of the mantis shrimps’ dactyl clubs.
CMP-113, a truncated fusion protein inspired by the CMP-1, was designed to
incorporate liquid-liquid phase separation (LLPS)-promoting motifs to investigate
how phase separation can potentially contribute to biomineralization.
In the presence of various Hofmeister salts, coacervation of CMP-113 was assessed
through brightfield microscopy, turbidity assays, dynamic light scattering (DLS)
measurements and nuclear magnetic resonance (NMR) spectroscopy. Its resistance
to 1,6-hexanediol treatment and re-entrant LLPS in presence of calcium chloride
suggests that LLPS is not purely hydrophobic-driven but may potentially electrostatic
interactions as well. Morphological observations of crystals formed in
biomineralization experiments not only demonstrated CMP-113’s capability to form
hydroxyapatite from solution, but its coacervation may also be imperative for
accelerating crystal nucleation and growth. These findings were consistent with the
wider literature on non-classical nucleation pathways of biomineralization. Future
research on CMP-113 and its applications in biomaterials and materials science is
anticipated to unveil new insights and open up exciting opportunities in the field of
biomaterials research. |
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Konstantin Pervushin |
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Konstantin Pervushin Koh, Jing Han |
format |
Final Year Project |
author |
Koh, Jing Han |
author_sort |
Koh, Jing Han |
title |
Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
title_short |
Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
title_full |
Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
title_fullStr |
Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
title_full_unstemmed |
Investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
title_sort |
investigating phase separation and mineralisation mechanics of mantis shrimp-derived fusion proteins |
publisher |
Nanyang Technological University |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/167170 |
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1772826685519953920 |