Tracking nanoscopic particles with interferometer scattering microscopy

• Main Author: Wong, Kuan Hua
• Other Authors: Tong Ling
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2023
• Subjects: Engineering::Bioengineering
• Online Access: https://hdl.handle.net/10356/167405
• Institution: Nanyang Technological University

The characterization of particles is crucial in the biomedical research field and can be accomplished using a variety of imaging modalities and detection methods. Single particle tracking (SPT) is a technique that enables the study of nanoscale objects' trajectories, but its fluorescence-based implementation has limited spatiotemporal resolution due to various factors. On the other hand, interferometric scattering (iSCAT) microscopy is a non-fluorescent interference-based imaging technique that can achieve highly sensitive detection of nanoscale objects and investigate their dynamics at the nanoscale level. SPT based on iSCAT provides high spatiotemporal resolution, with limitations only imposed by the intrinsic properties of the detection system and shot noise. In this project, we present an image processing and data analysis pipeline that utilizes the PiSCAT library and trackpy toolkit to analyze iSCAT images and obtain quantitative information on nanoparticles’ trajectories. Our findings indicate that the proposed method successfully locates the nanoparticles and obtains trajectory data for both datasets, which shows the binding interaction consist of two different modified gold nanoparticles (AuNPs) with varying target concentrations.