Denaturants effect on E2 protein

• Main Author: Ratna Ekawati Dharmawan
• Other Authors: Lim Sierin
• Format: Final Year Project
• Language: English
• Published: 2009
• Subjects: DRNTU::Engineering::Chemical engineering::Biotechnological production
• Online Access: http://hdl.handle.net/10356/16871
• Institution: Nanyang Technological University

The self-assembly of a protein cage composed of the catalytic core domain of pyruvate dehydrogenase multienzyme complex (E2) was investigated. E2 was produced in Escherichia coli (BL21 (DE3)). The hydrodynamic size of E2 was measured to be 27-28 nm using Dynamic Light Scattering technique. E2 was su...