Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation

In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic...

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Main Authors: Zhang, Liang, Wang, Xiuping, Chen, Yun, Zhang, Baorui, Xu, Hui, Li, Chenchen, Zhou, Yan
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/169014
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1690142023-06-27T02:29:26Z Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation Zhang, Liang Wang, Xiuping Chen, Yun Zhang, Baorui Xu, Hui Li, Chenchen Zhou, Yan School of Civil and Environmental Engineering Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute Engineering::Environmental engineering Anaerobic Fermentation Thermal Hydrolysis Process In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic fermentation of mixed sludge without thermal hydrolysis process (THP) pretreatment. THP increased the MCFA production by approximately 128% in the anaerobic fermentation. During 102 days of operation, the fermentation of THP pre-treated mixed sludge stably generated 2.9 g COD/L MCFAs. The self-generated EDs could not maximize MCFA production, and external addition of ethanol improved MCFA yield. Caproiciproducens was the dominant chain-elongating bacteria. PICRUST2 revealed that both fatty acid biosynthesis and reverse β-oxidation pathways could participate in MCFA synthesis, and ethanol addition could enhance the contribution of the reverse β-oxidation pathway. Future studies should focus on the improvement of MCFA production from THP-assisted sludge fermentation. Nanyang Technological University The authors acknowledge the support from Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University. 2023-06-27T02:29:26Z 2023-06-27T02:29:26Z 2023 Journal Article Zhang, L., Wang, X., Chen, Y., Zhang, B., Xu, H., Li, C. & Zhou, Y. (2023). Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation. Bioresource Technology, 374, 128805-. https://dx.doi.org/10.1016/j.biortech.2023.128805 0960-8524 https://hdl.handle.net/10356/169014 10.1016/j.biortech.2023.128805 36849100 2-s2.0-85149277859 374 128805 en Bioresource Technology © 2023 Elsevier Ltd. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Environmental engineering
Anaerobic Fermentation
Thermal Hydrolysis Process
spellingShingle Engineering::Environmental engineering
Anaerobic Fermentation
Thermal Hydrolysis Process
Zhang, Liang
Wang, Xiuping
Chen, Yun
Zhang, Baorui
Xu, Hui
Li, Chenchen
Zhou, Yan
Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
description In this study, medium-chain fatty acid (MCFA) generation from mixed sludge (including primary sludge and waste activated sludge) was investigated without additional electron donors (EDs). 0.5 g COD/L of MCFAs was produced and the in situ generated ethanol could serve as the EDs during the anaerobic fermentation of mixed sludge without thermal hydrolysis process (THP) pretreatment. THP increased the MCFA production by approximately 128% in the anaerobic fermentation. During 102 days of operation, the fermentation of THP pre-treated mixed sludge stably generated 2.9 g COD/L MCFAs. The self-generated EDs could not maximize MCFA production, and external addition of ethanol improved MCFA yield. Caproiciproducens was the dominant chain-elongating bacteria. PICRUST2 revealed that both fatty acid biosynthesis and reverse β-oxidation pathways could participate in MCFA synthesis, and ethanol addition could enhance the contribution of the reverse β-oxidation pathway. Future studies should focus on the improvement of MCFA production from THP-assisted sludge fermentation.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Zhang, Liang
Wang, Xiuping
Chen, Yun
Zhang, Baorui
Xu, Hui
Li, Chenchen
Zhou, Yan
format Article
author Zhang, Liang
Wang, Xiuping
Chen, Yun
Zhang, Baorui
Xu, Hui
Li, Chenchen
Zhou, Yan
author_sort Zhang, Liang
title Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
title_short Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
title_full Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
title_fullStr Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
title_full_unstemmed Medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
title_sort medium-chain fatty acid production from thermal hydrolysed sludge without external electron donor supplementation
publishDate 2023
url https://hdl.handle.net/10356/169014
_version_ 1772826240016711680