Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles

This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of...

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Main Authors: Rasouli, Elisa, Basirun, Wan Jefrey, Johan, Mohd Rafie, Rezayi, Majid, Mahmoudian, Mohammad Reza, Poenar, Daniel Puiu
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/169276
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1692762023-07-14T15:39:44Z Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles Rasouli, Elisa Basirun, Wan Jefrey Johan, Mohd Rafie Rezayi, Majid Mahmoudian, Mohammad Reza Poenar, Daniel Puiu School of Electrical and Electronic Engineering Continental-NTU Corporate Lab Engineering::Electrical and electronic engineering DNA Biosensor Cervical Cancer This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of the electrodes were then functionalized with thiolated single-strand DNA (ssDNA) probe human papillomavirus (HPV) DNA sequences. Next, the hybridization events with the immobilized probe DNA were monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using [Fe(CN)6]3‐/4− as the redox indicator. Our results demonstrate that the modified electrodes could distinguish the redox current signals of [Fe(CN)6] 3−/4− due to the absence/presence of the immobilized probe DNA. Furthermore, quantitative estimations of the concentration of the probe DNA were also possible. Optimal performance was obtained for probe DNA concentrations between 1 and 10 μM. The best performance of our HPV biosensor was obtained for probe DNA concentration of 5 μM, for which the limit of detection and sensitivity of our developed sensor resulted to be 0.1 nM and 2.4 μA/nM, respectively. Nanyang Technological University Published version This work was supported by University Malaya under the RU001- 2020 Research grant, Nanyang Technological University under the RIE2020 Industry Alignment Fund–Industry Collaboration Projects (IAF-ICP) Funding Initiative, as well as cash and in-kind contribution from the industry partner(s). 2023-07-11T02:06:59Z 2023-07-11T02:06:59Z 2023 Journal Article Rasouli, E., Basirun, W. J., Johan, M. R., Rezayi, M., Mahmoudian, M. R. & Poenar, D. P. (2023). Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles. Sensing and Bio-Sensing Research, 40, 100562-. https://dx.doi.org/10.1016/j.sbsr.2023.100562 2214-1804 https://hdl.handle.net/10356/169276 10.1016/j.sbsr.2023.100562 2-s2.0-85152120697 40 100562 en IAF-ICP Sensing and Bio-Sensing Research © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Electrical and electronic engineering
DNA Biosensor
Cervical Cancer
spellingShingle Engineering::Electrical and electronic engineering
DNA Biosensor
Cervical Cancer
Rasouli, Elisa
Basirun, Wan Jefrey
Johan, Mohd Rafie
Rezayi, Majid
Mahmoudian, Mohammad Reza
Poenar, Daniel Puiu
Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
description This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of the electrodes were then functionalized with thiolated single-strand DNA (ssDNA) probe human papillomavirus (HPV) DNA sequences. Next, the hybridization events with the immobilized probe DNA were monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using [Fe(CN)6]3‐/4− as the redox indicator. Our results demonstrate that the modified electrodes could distinguish the redox current signals of [Fe(CN)6] 3−/4− due to the absence/presence of the immobilized probe DNA. Furthermore, quantitative estimations of the concentration of the probe DNA were also possible. Optimal performance was obtained for probe DNA concentrations between 1 and 10 μM. The best performance of our HPV biosensor was obtained for probe DNA concentration of 5 μM, for which the limit of detection and sensitivity of our developed sensor resulted to be 0.1 nM and 2.4 μA/nM, respectively.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Rasouli, Elisa
Basirun, Wan Jefrey
Johan, Mohd Rafie
Rezayi, Majid
Mahmoudian, Mohammad Reza
Poenar, Daniel Puiu
format Article
author Rasouli, Elisa
Basirun, Wan Jefrey
Johan, Mohd Rafie
Rezayi, Majid
Mahmoudian, Mohammad Reza
Poenar, Daniel Puiu
author_sort Rasouli, Elisa
title Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
title_short Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
title_full Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
title_fullStr Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
title_full_unstemmed Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
title_sort electrochemical dna-nano biosensor for the detection of cervical cancer-causing hpv-16 using ultrasmall fe₃o₄-au core-shell nanoparticles
publishDate 2023
url https://hdl.handle.net/10356/169276
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