Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles
This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of...
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sg-ntu-dr.10356-1692762023-07-14T15:39:44Z Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles Rasouli, Elisa Basirun, Wan Jefrey Johan, Mohd Rafie Rezayi, Majid Mahmoudian, Mohammad Reza Poenar, Daniel Puiu School of Electrical and Electronic Engineering Continental-NTU Corporate Lab Engineering::Electrical and electronic engineering DNA Biosensor Cervical Cancer This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of the electrodes were then functionalized with thiolated single-strand DNA (ssDNA) probe human papillomavirus (HPV) DNA sequences. Next, the hybridization events with the immobilized probe DNA were monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using [Fe(CN)6]3‐/4− as the redox indicator. Our results demonstrate that the modified electrodes could distinguish the redox current signals of [Fe(CN)6] 3−/4− due to the absence/presence of the immobilized probe DNA. Furthermore, quantitative estimations of the concentration of the probe DNA were also possible. Optimal performance was obtained for probe DNA concentrations between 1 and 10 μM. The best performance of our HPV biosensor was obtained for probe DNA concentration of 5 μM, for which the limit of detection and sensitivity of our developed sensor resulted to be 0.1 nM and 2.4 μA/nM, respectively. Nanyang Technological University Published version This work was supported by University Malaya under the RU001- 2020 Research grant, Nanyang Technological University under the RIE2020 Industry Alignment Fund–Industry Collaboration Projects (IAF-ICP) Funding Initiative, as well as cash and in-kind contribution from the industry partner(s). 2023-07-11T02:06:59Z 2023-07-11T02:06:59Z 2023 Journal Article Rasouli, E., Basirun, W. J., Johan, M. R., Rezayi, M., Mahmoudian, M. R. & Poenar, D. P. (2023). Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles. Sensing and Bio-Sensing Research, 40, 100562-. https://dx.doi.org/10.1016/j.sbsr.2023.100562 2214-1804 https://hdl.handle.net/10356/169276 10.1016/j.sbsr.2023.100562 2-s2.0-85152120697 40 100562 en IAF-ICP Sensing and Bio-Sensing Research © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). application/pdf |
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Engineering::Electrical and electronic engineering DNA Biosensor Cervical Cancer Rasouli, Elisa Basirun, Wan Jefrey Johan, Mohd Rafie Rezayi, Majid Mahmoudian, Mohammad Reza Poenar, Daniel Puiu Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles |
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This paper reports a label-free biosensor for detecting human papillomavirus type 16 (HPV-16). For this purpose, the surface of the screen-printed carbon electrodes (SPCEs) was coated with Fe3O4-Au core-shell nanoparticles (NPs) using a green and facile eco-friendly method. The modified surfaces of the electrodes were then functionalized with thiolated single-strand DNA (ssDNA) probe human papillomavirus (HPV) DNA sequences. Next, the hybridization events with the immobilized probe DNA were monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using [Fe(CN)6]3‐/4− as the redox indicator. Our results demonstrate that the modified electrodes could distinguish the redox current signals of [Fe(CN)6] 3−/4− due to the absence/presence of the immobilized probe DNA. Furthermore, quantitative estimations of the concentration of the probe DNA were also possible. Optimal performance was obtained for probe DNA concentrations between 1 and 10 μM. The best performance of our HPV biosensor was obtained for probe DNA concentration of 5 μM, for which the limit of detection and sensitivity of our developed sensor resulted to be 0.1 nM and 2.4 μA/nM, respectively. |
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School of Electrical and Electronic Engineering |
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School of Electrical and Electronic Engineering Rasouli, Elisa Basirun, Wan Jefrey Johan, Mohd Rafie Rezayi, Majid Mahmoudian, Mohammad Reza Poenar, Daniel Puiu |
format |
Article |
author |
Rasouli, Elisa Basirun, Wan Jefrey Johan, Mohd Rafie Rezayi, Majid Mahmoudian, Mohammad Reza Poenar, Daniel Puiu |
author_sort |
Rasouli, Elisa |
title |
Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles |
title_short |
Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles |
title_full |
Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles |
title_fullStr |
Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles |
title_full_unstemmed |
Electrochemical DNA-nano biosensor for the detection of cervical cancer-causing HPV-16 using ultrasmall Fe₃O₄-Au core-shell nanoparticles |
title_sort |
electrochemical dna-nano biosensor for the detection of cervical cancer-causing hpv-16 using ultrasmall fe₃o₄-au core-shell nanoparticles |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/169276 |
_version_ |
1772828891474296832 |