In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic

• Main Authors: Phua, Rachel, Tien, Tracy Zhijun, Goh, Gracia, Pantelireis, Nikolaos, Sim, Kenice Siew Hoon, Lim, Jamien Wee Han, Clavel, Carlos
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Medicine; Cell Culture; Developmental Biology
• Online Access: https://hdl.handle.net/10356/169635
• Institution: Nanyang Technological University

Here, we present a protocol to set up and study 2D keratinocyte-melanocyte co-cultures and 3D full-thickness human skin equivalents. We describe steps for culturing of keratinocyte and melanocyte lines and the establishment of both 2D and 3D co-cultures. The cultures are utilized to measure melanin...