Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
Digital information, when converted into a DNA sequence, provides dense, stable, energy-efficient, and sustainable data storage. The most stable method for encapsulating DNA has been in an inorganic matrix of silica, iron oxide, or both, but are limited by low DNA uptake and complex recovery techniq...
Saved in:
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2023
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/169849 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-169849 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-1698492023-08-11T15:43:54Z Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage Fei, Zhongjie Gupta, Nupur Li, Mengjie Xiao, Pengfeng Hu, Xiao School of Materials Science and Engineering Interdisciplinary Graduate School (IGS) Nanyang Environment and Water Research Institute Engineering::Materials Digital Information DNA Complex Digital information, when converted into a DNA sequence, provides dense, stable, energy-efficient, and sustainable data storage. The most stable method for encapsulating DNA has been in an inorganic matrix of silica, iron oxide, or both, but are limited by low DNA uptake and complex recovery techniques. This study investigated a rationally designed thermally responsive functionally graded (TRFG) hydrogel as a simple and cost-effective method for storing DNA. The TRFG hydrogel shows high DNA uptake, long-term protection, and reusability due to nondestructive DNA extraction. The high loading capacity was achieved by directly absorbing DNA from the solution, which is then retained because of its interaction with a hyperbranched cationic polymer loaded into a negatively charged hydrogel matrix used as a support and because of its thermoresponsive nature, which allows DNA concentration within the hydrogel through multiple swelling/deswelling cycles. We were able to achieve a high DNA data density of 7.0 × 109 gigabytes per gram using a hydrogel-based system. Published version This work was supported by the National Key Research and Development Program of China (no. 2020YFA0712104) and the National Natural Science Foundation of China (no. 61971123). 2023-08-08T01:23:55Z 2023-08-08T01:23:55Z 2023 Journal Article Fei, Z., Gupta, N., Li, M., Xiao, P. & Hu, X. (2023). Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage. Science Advances, 9(19), eadg9933-. https://dx.doi.org/10.1126/sciadv.adg9933 2375-2548 https://hdl.handle.net/10356/169849 10.1126/sciadv.adg9933 37163589 2-s2.0-85158860167 19 9 eadg9933 en Science Advances © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Engineering::Materials Digital Information DNA Complex |
spellingShingle |
Engineering::Materials Digital Information DNA Complex Fei, Zhongjie Gupta, Nupur Li, Mengjie Xiao, Pengfeng Hu, Xiao Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage |
description |
Digital information, when converted into a DNA sequence, provides dense, stable, energy-efficient, and sustainable data storage. The most stable method for encapsulating DNA has been in an inorganic matrix of silica, iron oxide, or both, but are limited by low DNA uptake and complex recovery techniques. This study investigated a rationally designed thermally responsive functionally graded (TRFG) hydrogel as a simple and cost-effective method for storing DNA. The TRFG hydrogel shows high DNA uptake, long-term protection, and reusability due to nondestructive DNA extraction. The high loading capacity was achieved by directly absorbing DNA from the solution, which is then retained because of its interaction with a hyperbranched cationic polymer loaded into a negatively charged hydrogel matrix used as a support and because of its thermoresponsive nature, which allows DNA concentration within the hydrogel through multiple swelling/deswelling cycles. We were able to achieve a high DNA data density of 7.0 × 109 gigabytes per gram using a hydrogel-based system. |
author2 |
School of Materials Science and Engineering |
author_facet |
School of Materials Science and Engineering Fei, Zhongjie Gupta, Nupur Li, Mengjie Xiao, Pengfeng Hu, Xiao |
format |
Article |
author |
Fei, Zhongjie Gupta, Nupur Li, Mengjie Xiao, Pengfeng Hu, Xiao |
author_sort |
Fei, Zhongjie |
title |
Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage |
title_short |
Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage |
title_full |
Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage |
title_fullStr |
Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage |
title_full_unstemmed |
Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage |
title_sort |
toward highly effective loading of dna in hydrogels for high-density and long-term information storage |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/169849 |
_version_ |
1779156599766515712 |