Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage

Digital information, when converted into a DNA sequence, provides dense, stable, energy-efficient, and sustainable data storage. The most stable method for encapsulating DNA has been in an inorganic matrix of silica, iron oxide, or both, but are limited by low DNA uptake and complex recovery techniq...

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Main Authors: Fei, Zhongjie, Gupta, Nupur, Li, Mengjie, Xiao, Pengfeng, Hu, Xiao
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/169849
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1698492023-08-11T15:43:54Z Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage Fei, Zhongjie Gupta, Nupur Li, Mengjie Xiao, Pengfeng Hu, Xiao School of Materials Science and Engineering Interdisciplinary Graduate School (IGS) Nanyang Environment and Water Research Institute Engineering::Materials Digital Information DNA Complex Digital information, when converted into a DNA sequence, provides dense, stable, energy-efficient, and sustainable data storage. The most stable method for encapsulating DNA has been in an inorganic matrix of silica, iron oxide, or both, but are limited by low DNA uptake and complex recovery techniques. This study investigated a rationally designed thermally responsive functionally graded (TRFG) hydrogel as a simple and cost-effective method for storing DNA. The TRFG hydrogel shows high DNA uptake, long-term protection, and reusability due to nondestructive DNA extraction. The high loading capacity was achieved by directly absorbing DNA from the solution, which is then retained because of its interaction with a hyperbranched cationic polymer loaded into a negatively charged hydrogel matrix used as a support and because of its thermoresponsive nature, which allows DNA concentration within the hydrogel through multiple swelling/deswelling cycles. We were able to achieve a high DNA data density of 7.0 × 109 gigabytes per gram using a hydrogel-based system. Published version This work was supported by the National Key Research and Development Program of China (no. 2020YFA0712104) and the National Natural Science Foundation of China (no. 61971123). 2023-08-08T01:23:55Z 2023-08-08T01:23:55Z 2023 Journal Article Fei, Z., Gupta, N., Li, M., Xiao, P. & Hu, X. (2023). Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage. Science Advances, 9(19), eadg9933-. https://dx.doi.org/10.1126/sciadv.adg9933 2375-2548 https://hdl.handle.net/10356/169849 10.1126/sciadv.adg9933 37163589 2-s2.0-85158860167 19 9 eadg9933 en Science Advances © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Digital Information
DNA Complex
spellingShingle Engineering::Materials
Digital Information
DNA Complex
Fei, Zhongjie
Gupta, Nupur
Li, Mengjie
Xiao, Pengfeng
Hu, Xiao
Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
description Digital information, when converted into a DNA sequence, provides dense, stable, energy-efficient, and sustainable data storage. The most stable method for encapsulating DNA has been in an inorganic matrix of silica, iron oxide, or both, but are limited by low DNA uptake and complex recovery techniques. This study investigated a rationally designed thermally responsive functionally graded (TRFG) hydrogel as a simple and cost-effective method for storing DNA. The TRFG hydrogel shows high DNA uptake, long-term protection, and reusability due to nondestructive DNA extraction. The high loading capacity was achieved by directly absorbing DNA from the solution, which is then retained because of its interaction with a hyperbranched cationic polymer loaded into a negatively charged hydrogel matrix used as a support and because of its thermoresponsive nature, which allows DNA concentration within the hydrogel through multiple swelling/deswelling cycles. We were able to achieve a high DNA data density of 7.0 × 109 gigabytes per gram using a hydrogel-based system.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Fei, Zhongjie
Gupta, Nupur
Li, Mengjie
Xiao, Pengfeng
Hu, Xiao
format Article
author Fei, Zhongjie
Gupta, Nupur
Li, Mengjie
Xiao, Pengfeng
Hu, Xiao
author_sort Fei, Zhongjie
title Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
title_short Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
title_full Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
title_fullStr Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
title_full_unstemmed Toward highly effective loading of DNA in hydrogels for high-density and long-term information storage
title_sort toward highly effective loading of dna in hydrogels for high-density and long-term information storage
publishDate 2023
url https://hdl.handle.net/10356/169849
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