A metagenomic survey of wood decay fungi in the urban trees of Singapore
Mature tropical urban trees are susceptible to root and trunk rot caused by pathogenic fungi. A metagenomic survey of such fungi was carried out on 210 soil and tissue samples collected from 134 trees of 14 common species in Singapore. Furthermore, 121 fruiting bodies were collected and barcoded. Ou...
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sg-ntu-dr.10356-1700292023-08-28T15:32:00Z A metagenomic survey of wood decay fungi in the urban trees of Singapore Hong, Yan Tan, Jhing Yein Xue, Huiyu Chow, Mei Lun Mohamed Ali Ng, Arthur Leong, Abigail Yeo, Jeb Koh, Shao Ming Tang, Megan Shi Ying Lee, Yan Yi Choong, Amy Mei Fun Lee, Serena Mei Lyn Delli Ponti, Riccardo Chan, Perry M. Lee, Daryl Wong, Jia Yih Mutwil, Marek Fong, Yok King School of Biological Sciences Science::Biological sciences Wood Rotting Fungi Metagenomics Mature tropical urban trees are susceptible to root and trunk rot caused by pathogenic fungi. A metagenomic survey of such fungi was carried out on 210 soil and tissue samples collected from 134 trees of 14 common species in Singapore. Furthermore, 121 fruiting bodies were collected and barcoded. Out of the 22,067 OTUs (operational taxonomic units) identified, 10,646 OTUs had annotation information, and most were either ascomycetes (63.4%) or basidiomycetes (22.5%). Based on their detection in the diseased tissues and surrounding soils and/or the presence of fruiting bodies, fourteen basidiomycetes (nine Polyporales, four Hymenochaetales, one Boletales) and three ascomycetes (three species of Scytalidium) were strongly associated with the diseased trees. Fulvifomes siamensis affected the largest number of tree species surveyed. The association of three fungi was further supported by in vitro wood decay studies. Genetic heterogeneity was common in the diseased tissues and fruiting bodies (Ganoderma species especially). This survey identified the common pathogenic fungi of tropical urban trees and laid the foundation for early diagnosis and targeted mitigation efforts. It also illustrated the complexity of fungal ecology and pathogenicity. Ministry of National Development (MND) Nanyang Technological University National Parks Board Published version This research was funded by The Ministry of National Development Research Fund/National Parks Board Singapore to Y.H., M.M., P.M.C. (grant number NTU REF 2019-1120). It was also partially supported by the School of Biological Sciences, Nanyang Technological University Final Year Project (FYP) funds for J.Y.T., S.M.K., M.S.Y.T. and Y.Y.L. 2023-08-22T04:23:21Z 2023-08-22T04:23:21Z 2023 Journal Article Hong, Y., Tan, J. Y., Xue, H., Chow, M. L., Mohamed Ali, Ng, A., Leong, A., Yeo, J., Koh, S. M., Tang, M. S. Y., Lee, Y. Y., Choong, A. M. F., Lee, S. M. L., Delli Ponti, R., Chan, P. M., Lee, D., Wong, J. Y., Mutwil, M. & Fong, Y. K. (2023). A metagenomic survey of wood decay fungi in the urban trees of Singapore. Journal of Fungi, 9(4), 460-. https://dx.doi.org/10.3390/jof9040460 2309-608X https://hdl.handle.net/10356/170029 10.3390/jof9040460 37108914 2-s2.0-85154063092 4 9 460 en NTU REF 2019-1120 Journal of Fungi © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
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Science::Biological sciences Wood Rotting Fungi Metagenomics Hong, Yan Tan, Jhing Yein Xue, Huiyu Chow, Mei Lun Mohamed Ali Ng, Arthur Leong, Abigail Yeo, Jeb Koh, Shao Ming Tang, Megan Shi Ying Lee, Yan Yi Choong, Amy Mei Fun Lee, Serena Mei Lyn Delli Ponti, Riccardo Chan, Perry M. Lee, Daryl Wong, Jia Yih Mutwil, Marek Fong, Yok King A metagenomic survey of wood decay fungi in the urban trees of Singapore |
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Mature tropical urban trees are susceptible to root and trunk rot caused by pathogenic fungi. A metagenomic survey of such fungi was carried out on 210 soil and tissue samples collected from 134 trees of 14 common species in Singapore. Furthermore, 121 fruiting bodies were collected and barcoded. Out of the 22,067 OTUs (operational taxonomic units) identified, 10,646 OTUs had annotation information, and most were either ascomycetes (63.4%) or basidiomycetes (22.5%). Based on their detection in the diseased tissues and surrounding soils and/or the presence of fruiting bodies, fourteen basidiomycetes (nine Polyporales, four Hymenochaetales, one Boletales) and three ascomycetes (three species of Scytalidium) were strongly associated with the diseased trees. Fulvifomes siamensis affected the largest number of tree species surveyed. The association of three fungi was further supported by in vitro wood decay studies. Genetic heterogeneity was common in the diseased tissues and fruiting bodies (Ganoderma species especially). This survey identified the common pathogenic fungi of tropical urban trees and laid the foundation for early diagnosis and targeted mitigation efforts. It also illustrated the complexity of fungal ecology and pathogenicity. |
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School of Biological Sciences |
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School of Biological Sciences Hong, Yan Tan, Jhing Yein Xue, Huiyu Chow, Mei Lun Mohamed Ali Ng, Arthur Leong, Abigail Yeo, Jeb Koh, Shao Ming Tang, Megan Shi Ying Lee, Yan Yi Choong, Amy Mei Fun Lee, Serena Mei Lyn Delli Ponti, Riccardo Chan, Perry M. Lee, Daryl Wong, Jia Yih Mutwil, Marek Fong, Yok King |
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Article |
author |
Hong, Yan Tan, Jhing Yein Xue, Huiyu Chow, Mei Lun Mohamed Ali Ng, Arthur Leong, Abigail Yeo, Jeb Koh, Shao Ming Tang, Megan Shi Ying Lee, Yan Yi Choong, Amy Mei Fun Lee, Serena Mei Lyn Delli Ponti, Riccardo Chan, Perry M. Lee, Daryl Wong, Jia Yih Mutwil, Marek Fong, Yok King |
author_sort |
Hong, Yan |
title |
A metagenomic survey of wood decay fungi in the urban trees of Singapore |
title_short |
A metagenomic survey of wood decay fungi in the urban trees of Singapore |
title_full |
A metagenomic survey of wood decay fungi in the urban trees of Singapore |
title_fullStr |
A metagenomic survey of wood decay fungi in the urban trees of Singapore |
title_full_unstemmed |
A metagenomic survey of wood decay fungi in the urban trees of Singapore |
title_sort |
metagenomic survey of wood decay fungi in the urban trees of singapore |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/170029 |
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1779156290541453312 |