Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence

The opportunistic pathogen Staphylococcus aureus has an extremely complex relationship with humans. While the bacteria can exist as a commensal in many, it can cause a wide range of diseases and infections when turned pathogenic. Its presence is a determinant of chronicity and poor prognosis in nume...

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Main Authors: Ong, Zi Xin, Kannan, Bavani, Becker, David Lawrence
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/170400
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1704002023-09-11T06:10:09Z Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence Ong, Zi Xin Kannan, Bavani Becker, David Lawrence Lee Kong Chian School of Medicine (LKCMedicine) Skin Research Institute, Singapore Nanyang Institute of Technology in Health and Medicine Science::Medicine Gene Function Discovery Transposon Mutant Library The opportunistic pathogen Staphylococcus aureus has an extremely complex relationship with humans. While the bacteria can exist as a commensal in many, it can cause a wide range of diseases and infections when turned pathogenic. Its presence is a determinant of chronicity and poor prognosis in numerous diseases, and its genomic plasticity causes S. aureus antimicrobial resistance to be one of the most dire contemporary medical problems to solve. Genetic manipulation of S. aureus has led to numerous findings that are vital in the fight against its pathogenesis. The utilisation of transposon mutant libraries for the systematic inspection of the S. aureus genome led to many landmark discoveries pertaining to the bacteria's pathogenicity, antimicrobial resistance acquisition, and virulence regulation. In this review, we describe mutant libraries, and their significant contributions, from various S. aureus strains created with commonly used transposons. The general workflow for the construction of libraries will be presented, along with a discussion of the challenges of undertaking the task of large-scale library construction. As the accessibility of transposon mutant library construction, screening, and analysis increases, this genetic tool could be further exploited in the study of the S. aureus genome. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) Nanyang Technological University The composition of this review was supported in part by the Ministry of Education (MOE) Tier-1 grant 2019-T1-001-126, Nanyang Technological University Interdisciplinary Graduate School, and the Agency for Science, Technology and Research (A*STAR) under its Industry Alignment Fund–Pre-Positioning Programme (IAF-PP) grant for The Skin Research Institute of Singapore, Phase 2: SRIS@Novena, H1701a0004. 2023-09-11T06:10:09Z 2023-09-11T06:10:09Z 2023 Journal Article Ong, Z. X., Kannan, B. & Becker, D. L. (2023). Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence. Critical Reviews in Microbiology, 49(3), 297-317. https://dx.doi.org/10.1080/1040841X.2022.2052794 1040-841X https://hdl.handle.net/10356/170400 10.1080/1040841X.2022.2052794 35438613 2-s2.0-85129298512 3 49 297 317 en 2019-T1-001-126 H1701a0004 Critical Reviews in Microbiology © 2022 Informa UK Limited, trading as Taylor & Francis Group. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Gene Function Discovery
Transposon Mutant Library
spellingShingle Science::Medicine
Gene Function Discovery
Transposon Mutant Library
Ong, Zi Xin
Kannan, Bavani
Becker, David Lawrence
Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence
description The opportunistic pathogen Staphylococcus aureus has an extremely complex relationship with humans. While the bacteria can exist as a commensal in many, it can cause a wide range of diseases and infections when turned pathogenic. Its presence is a determinant of chronicity and poor prognosis in numerous diseases, and its genomic plasticity causes S. aureus antimicrobial resistance to be one of the most dire contemporary medical problems to solve. Genetic manipulation of S. aureus has led to numerous findings that are vital in the fight against its pathogenesis. The utilisation of transposon mutant libraries for the systematic inspection of the S. aureus genome led to many landmark discoveries pertaining to the bacteria's pathogenicity, antimicrobial resistance acquisition, and virulence regulation. In this review, we describe mutant libraries, and their significant contributions, from various S. aureus strains created with commonly used transposons. The general workflow for the construction of libraries will be presented, along with a discussion of the challenges of undertaking the task of large-scale library construction. As the accessibility of transposon mutant library construction, screening, and analysis increases, this genetic tool could be further exploited in the study of the S. aureus genome.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Ong, Zi Xin
Kannan, Bavani
Becker, David Lawrence
format Article
author Ong, Zi Xin
Kannan, Bavani
Becker, David Lawrence
author_sort Ong, Zi Xin
title Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence
title_short Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence
title_full Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence
title_fullStr Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence
title_full_unstemmed Exploiting transposons in the study of Staphylococcus aureus pathogenesis and virulence
title_sort exploiting transposons in the study of staphylococcus aureus pathogenesis and virulence
publishDate 2023
url https://hdl.handle.net/10356/170400
_version_ 1779156673671200768