Determination of particle size distribution by image analysis software.

The project aims to determine the particle size distribution (PSD) of spray-dried particles using automated image analysis with ImageJ software, as well as to justify if image analysis using ImageJ is comparable to that obtained from a Particle Sizer (Malvern, Mastersizer 2000) based on laser diffra...

Full description

Saved in:
Bibliographic Details
Main Author: Wong, Esther Yi Ning.
Other Authors: Kunn Hadinoto Ong
Format: Final Year Project
Language:English
Published: 2009
Subjects:
Online Access:http://hdl.handle.net/10356/17126
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-17126
record_format dspace
spelling sg-ntu-dr.10356-171262023-03-03T15:32:03Z Determination of particle size distribution by image analysis software. Wong, Esther Yi Ning. Kunn Hadinoto Ong School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology The project aims to determine the particle size distribution (PSD) of spray-dried particles using automated image analysis with ImageJ software, as well as to justify if image analysis using ImageJ is comparable to that obtained from a Particle Sizer (Malvern, Mastersizer 2000) based on laser diffraction. Validation of automated particle analysis was done against manual particle measurements in ImageJ, as a reference standard. Comparisons between statistical parameters like mean volume diameter, dv and volume median diameter, dv,50 showed the degree of accuracy. Comparison of standard deviation, σ, to further assess precision was carried out by an F-test. Number-based PSD were also plotted for each method. Results show that the Particle Sizer over-estimates particle size, resulting in significant differences when compared to image analysis. Automated image analysis works well for particles with little overlap using measurements of projected area diameter, dA, while manual measurements in ImageJ should be used to analyze heavily-agglomerated particles using measurements of Feret’s diameter, dFeret. Optimal thresholding methods for image segmentation were also found. Mixture modeling is suitable for bimodal gray-value distributions, and a local threshold is usually located at the valley or end of plateau before another peak. The problem of overlapping particles and agglomerates is also investigated to improve the accuracy of its image analysis. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2009-06-01T01:19:16Z 2009-06-01T01:19:16Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/17126 en Nanyang Technological University 81 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
Wong, Esther Yi Ning.
Determination of particle size distribution by image analysis software.
description The project aims to determine the particle size distribution (PSD) of spray-dried particles using automated image analysis with ImageJ software, as well as to justify if image analysis using ImageJ is comparable to that obtained from a Particle Sizer (Malvern, Mastersizer 2000) based on laser diffraction. Validation of automated particle analysis was done against manual particle measurements in ImageJ, as a reference standard. Comparisons between statistical parameters like mean volume diameter, dv and volume median diameter, dv,50 showed the degree of accuracy. Comparison of standard deviation, σ, to further assess precision was carried out by an F-test. Number-based PSD were also plotted for each method. Results show that the Particle Sizer over-estimates particle size, resulting in significant differences when compared to image analysis. Automated image analysis works well for particles with little overlap using measurements of projected area diameter, dA, while manual measurements in ImageJ should be used to analyze heavily-agglomerated particles using measurements of Feret’s diameter, dFeret. Optimal thresholding methods for image segmentation were also found. Mixture modeling is suitable for bimodal gray-value distributions, and a local threshold is usually located at the valley or end of plateau before another peak. The problem of overlapping particles and agglomerates is also investigated to improve the accuracy of its image analysis.
author2 Kunn Hadinoto Ong
author_facet Kunn Hadinoto Ong
Wong, Esther Yi Ning.
format Final Year Project
author Wong, Esther Yi Ning.
author_sort Wong, Esther Yi Ning.
title Determination of particle size distribution by image analysis software.
title_short Determination of particle size distribution by image analysis software.
title_full Determination of particle size distribution by image analysis software.
title_fullStr Determination of particle size distribution by image analysis software.
title_full_unstemmed Determination of particle size distribution by image analysis software.
title_sort determination of particle size distribution by image analysis software.
publishDate 2009
url http://hdl.handle.net/10356/17126
_version_ 1759853051845279744