Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique

• Main Authors: Han, Ho Le, Nurcahyanto, Dian Alfian, Muhammad, Neak, Lee, Yong-Jae, Nguyen, Tra T. H., Kim, Song-Gun, Chan, Sook Sin, Khoo, Kuan Shiong, Chew, Kit Wayne, Show, Pau Loke, Tran, Thi Ngoc Thu, Nguyen, Thi Dong Phuong, Chiu, Chen Yaw
• Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
• Format: Article
• Language: English
• Published: 2023
• Subjects: Engineering::Bioengineering; Bacteroidetes; Flexibacteraceae
• Online Access: https://hdl.handle.net/10356/171526
• Institution: Nanyang Technological University

In the effort of isolating novel microbial species, the strain PL0132T was isolated from a fallen leaf under fresh water at a stream, which glided when grown on a tap water medium (without nutrients). The strain was determined to be Gram-negative, strictly aerobic, and rod-shaped, which grew optimally at 25 °C, pH 6-7, and the strain tolerates 1% (w/v) NaCl concentration. The complete genome of strain PL0132T comprises one contig with a sequencing depth of 76×, consisting of 8,853,064 base pairs and the genomic DNA G + C content was 46.7% (genome). 16S rRNA gene sequence analysis revealed that strain PL0132T represents a member of the phylum Bacteroidetes and is affiliated with the genus Spirosoma. Based on genomic, phenotypic, and chemotaxonomic characteristics, the strain PL0132T represents a novel species of the genus Spirosoma, for which the name Spirosoma foliorum sp. nov. is proposed (= KCTC 72228 T = InaCC B1447T).