Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice
Background: Allergic asthma, one of the most common types of asthma, is thought to be highly susceptible to respiratory viral infections; however, its pathological mechanism needs to be elucidated. Recent studies have found impaired T-cell function in asthmatic mice. Therefore, we aimed to investiga...
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sg-ntu-dr.10356-1715342023-11-05T15:39:54Z Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice Ahn, So Yeon Lee, Jueun Lee, Dong-Ha Ho, Thi Len Le, Chau Thuy Tien Ko, Eun-Ju Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Allergic Asthma Ovalbumin Background: Allergic asthma, one of the most common types of asthma, is thought to be highly susceptible to respiratory viral infections; however, its pathological mechanism needs to be elucidated. Recent studies have found impaired T-cell function in asthmatic mice. Therefore, we aimed to investigate the way by which asthma induction affects T-cell exhaustion in the lungs and assess the relationship between T-cell exhaustion and influenza viral infection. Methods: Chronic allergic asthma mice were induced by intranasal injection of ovalbumin for 6 weeks and asthmatic features and T cell populations in lung or airway were assessed. To determine the influenza virus susceptibility, control and asthma mice were challenged with the human influenza virus strain A/Puerto Rico/8/1934 H1N1 and evaluated the survival rate, lung damage, and virus titer. Results: Six weeks of OVA sensitization and challenge successfully induced chronic allergic asthma in a mouse model showing significant increase of sera IgE level and broncho-pathological features. A significant decrease in interferon-γ-producing T-cell populations and an increase in exhausted T-cell populations in the lungs of OVA-induced asthmatic mice were observed. Asthmatic mice were more susceptible to influenza virus infection than control mice showing lower survival rate and higher virus titer in lung, and a positive correlation existed between T-cell exhaustion in the lung and virus titer. Conclusions: Asthma induction in mice results in the exhaustion of T-cell immunity, which may contribute to the defective capacity of viral protection. This study demonstrates a correlation between asthma conditions and viral susceptibility by investigating the functional characteristics of T-cells in asthma. Our results provide insights into the development of strategies to overcome the dangers of respiratory viral disease in patients with asthma. Published version This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2020R1F1A1073040) and Basic Science Research Program to Research Institute for Basic Sciences (RIBS) of Jeju National University through the NRF funded by the Ministry of Education (2019R1A6A1A10072987). 2023-10-30T01:48:34Z 2023-10-30T01:48:34Z 2023 Journal Article Ahn, S. Y., Lee, J., Lee, D., Ho, T. L., Le, C. T. T. & Ko, E. (2023). Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice. Respiratory Research, 24(1), 160-. https://dx.doi.org/10.1186/s12931-023-02448-9 1465-9921 https://hdl.handle.net/10356/171534 10.1186/s12931-023-02448-9 37424011 2-s2.0-85164203491 1 24 160 en Respiratory Research © The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. application/pdf |
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Science::Medicine Allergic Asthma Ovalbumin Ahn, So Yeon Lee, Jueun Lee, Dong-Ha Ho, Thi Len Le, Chau Thuy Tien Ko, Eun-Ju Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice |
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Background: Allergic asthma, one of the most common types of asthma, is thought to be highly susceptible to respiratory viral infections; however, its pathological mechanism needs to be elucidated. Recent studies have found impaired T-cell function in asthmatic mice. Therefore, we aimed to investigate the way by which asthma induction affects T-cell exhaustion in the lungs and assess the relationship between T-cell exhaustion and influenza viral infection. Methods: Chronic allergic asthma mice were induced by intranasal injection of ovalbumin for 6 weeks and asthmatic features and T cell populations in lung or airway were assessed. To determine the influenza virus susceptibility, control and asthma mice were challenged with the human influenza virus strain A/Puerto Rico/8/1934 H1N1 and evaluated the survival rate, lung damage, and virus titer. Results: Six weeks of OVA sensitization and challenge successfully induced chronic allergic asthma in a mouse model showing significant increase of sera IgE level and broncho-pathological features. A significant decrease in interferon-γ-producing T-cell populations and an increase in exhausted T-cell populations in the lungs of OVA-induced asthmatic mice were observed. Asthmatic mice were more susceptible to influenza virus infection than control mice showing lower survival rate and higher virus titer in lung, and a positive correlation existed between T-cell exhaustion in the lung and virus titer. Conclusions: Asthma induction in mice results in the exhaustion of T-cell immunity, which may contribute to the defective capacity of viral protection. This study demonstrates a correlation between asthma conditions and viral susceptibility by investigating the functional characteristics of T-cells in asthma. Our results provide insights into the development of strategies to overcome the dangers of respiratory viral disease in patients with asthma. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Ahn, So Yeon Lee, Jueun Lee, Dong-Ha Ho, Thi Len Le, Chau Thuy Tien Ko, Eun-Ju |
format |
Article |
author |
Ahn, So Yeon Lee, Jueun Lee, Dong-Ha Ho, Thi Len Le, Chau Thuy Tien Ko, Eun-Ju |
author_sort |
Ahn, So Yeon |
title |
Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice |
title_short |
Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice |
title_full |
Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice |
title_fullStr |
Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice |
title_full_unstemmed |
Chronic allergic asthma induces T-cell exhaustion and impairs virus clearance in mice |
title_sort |
chronic allergic asthma induces t-cell exhaustion and impairs virus clearance in mice |
publishDate |
2023 |
url |
https://hdl.handle.net/10356/171534 |
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1783955633505042432 |