Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore

Staphylococcus aureus contamination of food and food contact surfaces is a public health concern given its virulent and antimicrobial-resistant properties worldwide. In this study, a total of 181 MSSA isolates were analyzed for SE genes, antimicrobial resistance patterns, and spa types. Overall, 24....

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Main Authors: Lim, Ker Li, Khor, Wei Ching, Ong, Kar Hui, Timothy, Lois, Aung, Kyaw Thu
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/171717
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1717172023-11-06T15:32:06Z Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore Lim, Ker Li Khor, Wei Ching Ong, Kar Hui Timothy, Lois Aung, Kyaw Thu School of Biological Sciences Singapore Food Agency Science::Biological sciences Staphylococcus Aureus Antimicrobial Resistance Staphylococcus aureus contamination of food and food contact surfaces is a public health concern given its virulent and antimicrobial-resistant properties worldwide. In this study, a total of 181 MSSA isolates were analyzed for SE genes, antimicrobial resistance patterns, and spa types. Overall, 24.9% of isolates were positive for SE gene detection, with sea being the most prevalent classical SE (18.8%). The most predominant sample sources for SE gene contamination were hand swabs for sea (6/48), meat dishes for seb (3/14) and seafood dishes for sec (2/24). Antimicrobial resistance was also observed at relatively high frequencies for the clinically important antibiotics penicillin G and ampicillin (both 54.7%), followed by tetracycline (14.9%) and azithromycin (8.8%). In addition, characterization of spa types revealed spa type t5078 to be the most predominant (40.3%), with significant associations between spa types t127 and t5521 and the sea gene. This study offers insights into the enterotoxin gene and antimicrobial resistance profiles of S. aureus in cooked or ready-to-eat food to inform future surveillance and epidemiological studies. National Environmental Agency (NEA) Singapore Food Agency Published version This study was supported by the National Environment Agency and the Singapore Food Agency, Singapore. 2023-11-06T03:55:33Z 2023-11-06T03:55:33Z 2023 Journal Article Lim, K. L., Khor, W. C., Ong, K. H., Timothy, L. & Aung, K. T. (2023). Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore. Microorganisms, 11(7), 1785-. https://dx.doi.org/10.3390/microorganisms11071785 2076-2607 https://hdl.handle.net/10356/171717 10.3390/microorganisms11071785 37512957 2-s2.0-85166172678 7 11 1785 en Microorganisms © 2023 The authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Staphylococcus Aureus
Antimicrobial Resistance
spellingShingle Science::Biological sciences
Staphylococcus Aureus
Antimicrobial Resistance
Lim, Ker Li
Khor, Wei Ching
Ong, Kar Hui
Timothy, Lois
Aung, Kyaw Thu
Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore
description Staphylococcus aureus contamination of food and food contact surfaces is a public health concern given its virulent and antimicrobial-resistant properties worldwide. In this study, a total of 181 MSSA isolates were analyzed for SE genes, antimicrobial resistance patterns, and spa types. Overall, 24.9% of isolates were positive for SE gene detection, with sea being the most prevalent classical SE (18.8%). The most predominant sample sources for SE gene contamination were hand swabs for sea (6/48), meat dishes for seb (3/14) and seafood dishes for sec (2/24). Antimicrobial resistance was also observed at relatively high frequencies for the clinically important antibiotics penicillin G and ampicillin (both 54.7%), followed by tetracycline (14.9%) and azithromycin (8.8%). In addition, characterization of spa types revealed spa type t5078 to be the most predominant (40.3%), with significant associations between spa types t127 and t5521 and the sea gene. This study offers insights into the enterotoxin gene and antimicrobial resistance profiles of S. aureus in cooked or ready-to-eat food to inform future surveillance and epidemiological studies.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Lim, Ker Li
Khor, Wei Ching
Ong, Kar Hui
Timothy, Lois
Aung, Kyaw Thu
format Article
author Lim, Ker Li
Khor, Wei Ching
Ong, Kar Hui
Timothy, Lois
Aung, Kyaw Thu
author_sort Lim, Ker Li
title Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore
title_short Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore
title_full Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore
title_fullStr Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore
title_full_unstemmed Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore
title_sort occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in staphylococcus aureus in food and food contact surfaces in singapore
publishDate 2023
url https://hdl.handle.net/10356/171717
_version_ 1783955515527659520