Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress

Available evidence suggest that exposure to PM2.5 during pregnancy is associated with reduced cognitive function in offspring. This study aimed to investigate the effects of maternal exposure to PM2.5 on offspring cognitive function and to elucidate the underlying mechanisms. In this work, pregnant...

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Main Authors: Zhang, Jiajia, Yang, Yingying, Al-Ahmady, Zahraa S., Du, Wenchong, Duan, Jinjin, Liao, Zehuan, Sun, Qinghua, Wei, Zhiyun, Hua, Jing
Other Authors: School of Biological Sciences
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Language:English
Published: 2023
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Online Access:https://hdl.handle.net/10356/172162
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spelling sg-ntu-dr.10356-1721622023-12-04T15:31:59Z Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress Zhang, Jiajia Yang, Yingying Al-Ahmady, Zahraa S. Du, Wenchong Duan, Jinjin Liao, Zehuan Sun, Qinghua Wei, Zhiyun Hua, Jing School of Biological Sciences School of Social Sciences Science::Biological sciences Fine Particulate Matter Cognitive Impairment Available evidence suggest that exposure to PM2.5 during pregnancy is associated with reduced cognitive function in offspring. This study aimed to investigate the effects of maternal exposure to PM2.5 on offspring cognitive function and to elucidate the underlying mechanisms. In this work, pregnant C57BL/6 female mice were exposed to concentrated ambient PM2.5 or filtered air from day 0.5 (=vaginal plug) to day 15.5 in the Shanghai Meteorological and Environmental Animal Exposure System, and offspring cerebellar tissues were collected on embryonic day 15.5, as well as postnatal days 0, 10 and 42. The mean PM2.5 concentrations exposed to the pregnant mice were 73.06 ± 4.90 μg/m3 and 11.15 ± 2.71 μg/m3 in the concentrated ambient PM2.5 and filtered air chambers, respectively. Maternal concentrated PM2.5 exposure was negatively correlated with offspring spatial memory significantly as assessed by the Morris water maze. Compared with the filtered air group, PM2.5-exposed offspring mice had reduced cerebellar microglia. Both RNA and protein levels of IL-8 and TNF-α were elevated in the concentrated ambient PM2.5 group. PM2.5 exposure increased the level of 8-OHG in miRNA of microglia and Purkinje cells in 6-week-old offspring. The level of prostaglandin F2α (8-iso-PGF2Aα) in the cerebellum was increased at different growing stages of offspring after gestational exposure of PM2.5. These results suggested that maternal air pollution exposure might cause inflammatory damage and oxidative stress to the cerebellum, contributing to reduced cognitive performance in mice offspring. Published version This study was supported by the National Natural Science Foundation of China (81673179), the Science and Technology Commission of Shanghai Municipality (21DZ2202000, 19140903100), Shanghai Municipal Health Commission (2020YJZX0213), Pudong Municipal Health Commission (PW2020D-11) Multicenter Study on Accurate Evaluation and Prediction of Neurodevelopmental Retardation in Preterm Infants (Clinical Research Plan of SHDC, SHDC2020CR1047B). 2023-11-28T02:03:34Z 2023-11-28T02:03:34Z 2023 Journal Article Zhang, J., Yang, Y., Al-Ahmady, Z. S., Du, W., Duan, J., Liao, Z., Sun, Q., Wei, Z. & Hua, J. (2023). Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress. Ecotoxicology and Environmental Safety, 249, 114425-. https://dx.doi.org/10.1016/j.ecoenv.2022.114425 0147-6513 https://hdl.handle.net/10356/172162 10.1016/j.ecoenv.2022.114425 2-s2.0-85144268292 249 114425 en Ecotoxicology and Environmental Safety © 2022 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
Fine Particulate Matter
Cognitive Impairment
spellingShingle Science::Biological sciences
Fine Particulate Matter
Cognitive Impairment
Zhang, Jiajia
Yang, Yingying
Al-Ahmady, Zahraa S.
Du, Wenchong
Duan, Jinjin
Liao, Zehuan
Sun, Qinghua
Wei, Zhiyun
Hua, Jing
Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
description Available evidence suggest that exposure to PM2.5 during pregnancy is associated with reduced cognitive function in offspring. This study aimed to investigate the effects of maternal exposure to PM2.5 on offspring cognitive function and to elucidate the underlying mechanisms. In this work, pregnant C57BL/6 female mice were exposed to concentrated ambient PM2.5 or filtered air from day 0.5 (=vaginal plug) to day 15.5 in the Shanghai Meteorological and Environmental Animal Exposure System, and offspring cerebellar tissues were collected on embryonic day 15.5, as well as postnatal days 0, 10 and 42. The mean PM2.5 concentrations exposed to the pregnant mice were 73.06 ± 4.90 μg/m3 and 11.15 ± 2.71 μg/m3 in the concentrated ambient PM2.5 and filtered air chambers, respectively. Maternal concentrated PM2.5 exposure was negatively correlated with offspring spatial memory significantly as assessed by the Morris water maze. Compared with the filtered air group, PM2.5-exposed offspring mice had reduced cerebellar microglia. Both RNA and protein levels of IL-8 and TNF-α were elevated in the concentrated ambient PM2.5 group. PM2.5 exposure increased the level of 8-OHG in miRNA of microglia and Purkinje cells in 6-week-old offspring. The level of prostaglandin F2α (8-iso-PGF2Aα) in the cerebellum was increased at different growing stages of offspring after gestational exposure of PM2.5. These results suggested that maternal air pollution exposure might cause inflammatory damage and oxidative stress to the cerebellum, contributing to reduced cognitive performance in mice offspring.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Zhang, Jiajia
Yang, Yingying
Al-Ahmady, Zahraa S.
Du, Wenchong
Duan, Jinjin
Liao, Zehuan
Sun, Qinghua
Wei, Zhiyun
Hua, Jing
format Article
author Zhang, Jiajia
Yang, Yingying
Al-Ahmady, Zahraa S.
Du, Wenchong
Duan, Jinjin
Liao, Zehuan
Sun, Qinghua
Wei, Zhiyun
Hua, Jing
author_sort Zhang, Jiajia
title Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
title_short Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
title_full Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
title_fullStr Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
title_full_unstemmed Maternal exposure to PM₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
title_sort maternal exposure to pm₂.₅ induces cognitive impairment in offspring via cerebellar neuroinflammation and oxidative stress
publishDate 2023
url https://hdl.handle.net/10356/172162
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