Understanding Malassezia secreted proteases in host-microbial crosstalk in skin health

• Main Author: Goh, Joleen Pei Zhen
• Other Authors: David Lawrence Becker
• Format: Thesis-Doctor of Philosophy
• Language: English
• Published: Nanyang Technological University 2023
• Subjects: Science::Biological sciences::Microbiology
• Online Access: https://hdl.handle.net/10356/172557
• Institution: Nanyang Technological University

Malassezia form the dominant eukaryotic microbial community on the human skin. The Malassezia genus possesses a repertoire of secretory hydrolytic enzymes involved in protein and lipid metabolism which alter the external cutaneous environment. The exact role of most Malassezia secreted enzymes, including those in interaction with the epithelial surface, are not well characterized. In this study, we explored the functional roles of Malassezia aspartyl proteases in skin health, we generated a knockout mutant of the predominant aspartyl protease in the genetically tractable Malassezia furfur. We observed the loss of MFSAP1 resulted in dramatic changes in the cell adhesion and dispersal in both culture and a human 3D reconstituted epidermis model. In a murine model of Malassezia colonization, we further demonstrated Mfsap1 contributes to inflammation as observed by reduced edema and inflammatory cell infiltration with the knockout mutant versus wildtype. Taken together, we show that this dominant secretory Malassezia aspartyl protease has an important role in enabling a planktonic cellular state that can potentially aid in colonization and additionally as a virulence factor in barrier-compromised skin, highlighting the importance of considering the contextual relevance when evaluating the functions of secreted microbial enzymes.