Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration

The repair of bone defects using grafts is commonly employed in clinical practice. However, the risk of infection poses a significant concern. Tissue engineering scaffolds with antibacterial functionalities offer a better approach for bone tissue repair. In this work, firstly, two kinds of nanoparti...

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Main Authors: Zhang, Sihan, Zhao, Guanglei, Mahotra, Manish, Ma, Shiyuan, Li, Wenrui, Lee, Hiang Wee, Yu, Hong, Sampathkumar, Kaarunya, Xie, Denghui, Guo, Jinshan, Loo, Joachim Say Chye
Other Authors: School of Materials Science and Engineering
Format: Article
Language:English
Published: 2024
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Online Access:https://hdl.handle.net/10356/173058
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1730582024-01-12T15:47:36Z Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration Zhang, Sihan Zhao, Guanglei Mahotra, Manish Ma, Shiyuan Li, Wenrui Lee, Hiang Wee Yu, Hong Sampathkumar, Kaarunya Xie, Denghui Guo, Jinshan Loo, Joachim Say Chye School of Materials Science and Engineering Interdisciplinary Graduate School (IGS) Lee Kong Chian School of Medicine (LKCMedicine) NTU Institute for Health Technologies Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) Engineering::Materials Chitosan Nanofiber The repair of bone defects using grafts is commonly employed in clinical practice. However, the risk of infection poses a significant concern. Tissue engineering scaffolds with antibacterial functionalities offer a better approach for bone tissue repair. In this work, firstly, two kinds of nanoparticles were prepared using chitosan to complex with ciprofloxacin and BMP-2, respectively. The ciprofloxacin complex nanoparticles improved the dissolution efficiency of ciprofloxacin achieving a potent antibacterial effect and cumulative release reached 95 % in 7 h. For BMP-2 complexed nanoparticles, the release time points can be programmed at 80 h, 100 h or 180 h by regulating the number of coating chitosan layers. Secondly, a functional scaffold was prepared by combining the two nanoparticles with chitosan nanofibers. The microscopic nanofiber structure of the scaffold with 27.28 m2/g specific surface area promotes cell adhesion, high porosity provides space for cell growth, and facilitates drug loading and release. The multifunctional scaffold exhibits programmed release function, and has obvious antibacterial effect at the initial stage of implantation, and releases BMP-2 to promote osteogenic differentiation of mesenchymal stem cells after the antibacterial effect ends. The scaffold is expected to be applied in clinical bone repair and graft infection prevention. Ministry of Education (MOE) Singapore Food Agency Submitted/Accepted version The authors would like to acknowledge the financial support from the Singapore Centre for Environmental Life Sciences Engineering (SCELSE) (MOE/RCE: M4330019.C70), Ministry of Education AcRF-Tier 1 grant (RG19/18 and RT08/19), the Singapore National Biofilm Consortium (SNBC/2021/SF2/P04) and the Singapore Food Agency (SFS_RND_SUFP_001_06), National Natural Science Foundation of China (No. 81772315, 81974328) and Guangdong Science Foundation for Distinguished Young Scholars (No. 2022B1515020044), Guangxi Provincial Key Laboratory Funding of Bone and Joint Degeneration Diseases Basic and Translational Medicine. 2024-01-10T06:01:07Z 2024-01-10T06:01:07Z 2024 Journal Article Zhang, S., Zhao, G., Mahotra, M., Ma, S., Li, W., Lee, H. W., Yu, H., Sampathkumar, K., Xie, D., Guo, J. & Loo, J. S. C. (2024). Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration. International Journal of Biological Macromolecules, 254(Part 2), 127912-. https://dx.doi.org/10.1016/j.ijbiomac.2023.127912 0141-8130 https://hdl.handle.net/10356/173058 10.1016/j.ijbiomac.2023.127912 37939763 2-s2.0-85176260615 Part 2 254 127912 en MOE/RCE: M4330019.C70 RG19/18 RT08/19 SNBC/2021/SF2/P04 SFS_RND_SUFP_001_06 International Journal of Biological Macromolecules © 2023 Elsevier B.V. All rights reserved. This article may be downloaded for personal use only. Any other use requires prior permission of the copyright holder. The Version of Record is available online at http://doi.org/10.1016/j.ijbiomac.2023.127912. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Materials
Chitosan
Nanofiber
spellingShingle Engineering::Materials
Chitosan
Nanofiber
Zhang, Sihan
Zhao, Guanglei
Mahotra, Manish
Ma, Shiyuan
Li, Wenrui
Lee, Hiang Wee
Yu, Hong
Sampathkumar, Kaarunya
Xie, Denghui
Guo, Jinshan
Loo, Joachim Say Chye
Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration
description The repair of bone defects using grafts is commonly employed in clinical practice. However, the risk of infection poses a significant concern. Tissue engineering scaffolds with antibacterial functionalities offer a better approach for bone tissue repair. In this work, firstly, two kinds of nanoparticles were prepared using chitosan to complex with ciprofloxacin and BMP-2, respectively. The ciprofloxacin complex nanoparticles improved the dissolution efficiency of ciprofloxacin achieving a potent antibacterial effect and cumulative release reached 95 % in 7 h. For BMP-2 complexed nanoparticles, the release time points can be programmed at 80 h, 100 h or 180 h by regulating the number of coating chitosan layers. Secondly, a functional scaffold was prepared by combining the two nanoparticles with chitosan nanofibers. The microscopic nanofiber structure of the scaffold with 27.28 m2/g specific surface area promotes cell adhesion, high porosity provides space for cell growth, and facilitates drug loading and release. The multifunctional scaffold exhibits programmed release function, and has obvious antibacterial effect at the initial stage of implantation, and releases BMP-2 to promote osteogenic differentiation of mesenchymal stem cells after the antibacterial effect ends. The scaffold is expected to be applied in clinical bone repair and graft infection prevention.
author2 School of Materials Science and Engineering
author_facet School of Materials Science and Engineering
Zhang, Sihan
Zhao, Guanglei
Mahotra, Manish
Ma, Shiyuan
Li, Wenrui
Lee, Hiang Wee
Yu, Hong
Sampathkumar, Kaarunya
Xie, Denghui
Guo, Jinshan
Loo, Joachim Say Chye
format Article
author Zhang, Sihan
Zhao, Guanglei
Mahotra, Manish
Ma, Shiyuan
Li, Wenrui
Lee, Hiang Wee
Yu, Hong
Sampathkumar, Kaarunya
Xie, Denghui
Guo, Jinshan
Loo, Joachim Say Chye
author_sort Zhang, Sihan
title Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration
title_short Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration
title_full Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration
title_fullStr Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration
title_full_unstemmed Chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and BMP-2 nanoparticles for the conception of bone regeneration
title_sort chitosan nanofibrous scaffold with graded and controlled release of ciprofloxacin and bmp-2 nanoparticles for the conception of bone regeneration
publishDate 2024
url https://hdl.handle.net/10356/173058
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