Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies
Following the in vivo biodistribution of platelets can contribute to a better understanding of their physiological and pathological roles, and nuclear imaging methods, such as single photon emission tomography (SPECT), provide an excellent method for that. SPECT imaging needs stable labeling of the...
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sg-ntu-dr.10356-1737542024-03-03T15:38:35Z Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies Merkel, Keresztély Szöllősi, Dávid Horváth, Ildikó Jezsó, Bálint Baranyai, Zsolt Szigeti, Krisztián Varga, Zoltán Hegedüs, Imre Padmanabhan, Parasuraman Gulyás, Balázs Bergmann, Ralf Máthé, Domokos Lee Kong Chian School of Medicine (LKCMedicine) Medicine, Health and Life Sciences Platelet radiolabeling 99mTc-HYNIC-Duramycin Following the in vivo biodistribution of platelets can contribute to a better understanding of their physiological and pathological roles, and nuclear imaging methods, such as single photon emission tomography (SPECT), provide an excellent method for that. SPECT imaging needs stable labeling of the platelets with a radioisotope. In this study, we report a new method to label platelets with 99mTc, the most frequently used isotope for SPECT in clinical applications. The proposed radiolabeling procedure uses a membrane-binding peptide, duramycin. Our results show that duramycin does not cause significant platelet activation, and radiolabeling can be carried out with a procedure utilizing a simple labeling step followed by a size-exclusion chromatography-based purification step. The in vivo application of the radiolabeled human platelets in mice yielded quantitative biodistribution images of the spleen and liver and no accumulation in the lungs. The performed small-animal SPECT/CT in vivo imaging investigations revealed good in vivo stability of the labeling, which paves the way for further applications of 99mTc-labeled-Duramycin in platelet imaging. Published version Zoltán Varga was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences and the ÚNKP-21-5 Bolyai + New National Excellence Program of the Ministry for Innovation and Technology from the National Research, Development and Innovation Fund. Domokos Máthé received support from the European Union under project H2020-SmartAge, grant no. 859890. This work was supported by the European Union’s Horizon 2020 Research and Innovation Program, grant agreement no. 739593: HCEMM, supported by the EU Program H2020-EU.4.a. This work was also partly funded by grants from the Hungarian National Research, Development and Innovation Office (Thematic Excellence Program, TKP-BIOImaging, financed under the 2020–4.1.1- TKP2020 funding scheme, Investment to the Future 2020.1.16-Jöv˝o-2021–00013, TKP2021-EGA-31, and by KIM NKFIA 2022-2.1.1-NL-2022-00005 and KIM NKFIA TKP-2021-EGA-05). 2024-02-26T07:12:21Z 2024-02-26T07:12:21Z 2023 Journal Article Merkel, K., Szöllősi, D., Horváth, I., Jezsó, B., Baranyai, Z., Szigeti, K., Varga, Z., Hegedüs, I., Padmanabhan, P., Gulyás, B., Bergmann, R. & Máthé, D. (2023). Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies. International Journal of Molecular Sciences, 24(23), 17119-. https://dx.doi.org/10.3390/ijms242317119 1661-6596 https://hdl.handle.net/10356/173754 10.3390/ijms242317119 38069441 2-s2.0-85179307778 23 24 17119 en International Journal of Molecular Sciences © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). application/pdf |
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Medicine, Health and Life Sciences Platelet radiolabeling 99mTc-HYNIC-Duramycin |
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Medicine, Health and Life Sciences Platelet radiolabeling 99mTc-HYNIC-Duramycin Merkel, Keresztély Szöllősi, Dávid Horváth, Ildikó Jezsó, Bálint Baranyai, Zsolt Szigeti, Krisztián Varga, Zoltán Hegedüs, Imre Padmanabhan, Parasuraman Gulyás, Balázs Bergmann, Ralf Máthé, Domokos Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies |
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Following the in vivo biodistribution of platelets can contribute to a better understanding of their physiological and pathological roles, and nuclear imaging methods, such as single photon emission tomography (SPECT), provide an excellent method for that. SPECT imaging needs stable labeling of the platelets with a radioisotope. In this study, we report a new method to label platelets with 99mTc, the most frequently used isotope for SPECT in clinical applications. The proposed radiolabeling procedure uses a membrane-binding peptide, duramycin. Our results show that duramycin does not cause significant platelet activation, and radiolabeling can be carried out with a procedure utilizing a simple labeling step followed by a size-exclusion chromatography-based purification step. The in vivo application of the radiolabeled human platelets in mice yielded quantitative biodistribution images of the spleen and liver and no accumulation in the lungs. The performed small-animal SPECT/CT in vivo imaging investigations revealed good in vivo stability of the labeling, which paves the way for further applications of 99mTc-labeled-Duramycin in platelet imaging. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Merkel, Keresztély Szöllősi, Dávid Horváth, Ildikó Jezsó, Bálint Baranyai, Zsolt Szigeti, Krisztián Varga, Zoltán Hegedüs, Imre Padmanabhan, Parasuraman Gulyás, Balázs Bergmann, Ralf Máthé, Domokos |
format |
Article |
author |
Merkel, Keresztély Szöllősi, Dávid Horváth, Ildikó Jezsó, Bálint Baranyai, Zsolt Szigeti, Krisztián Varga, Zoltán Hegedüs, Imre Padmanabhan, Parasuraman Gulyás, Balázs Bergmann, Ralf Máthé, Domokos |
author_sort |
Merkel, Keresztély |
title |
Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies |
title_short |
Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies |
title_full |
Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies |
title_fullStr |
Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies |
title_full_unstemmed |
Radiolabeling of platelets with 99mTc-HYNIC-Duramycin for in vivo imaging studies |
title_sort |
radiolabeling of platelets with 99mtc-hynic-duramycin for in vivo imaging studies |
publishDate |
2024 |
url |
https://hdl.handle.net/10356/173754 |
_version_ |
1794549423001305088 |