Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye

Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, and permissible light fluen...

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Main Authors: Haindl, Richard, Bellemo, Valentina, Rajendran, Praveenbalaji, Tan, Bingyao, Liu, Mengyang, Lee, Beishi, Zhou, Qifa, Leitgeb, Rainer A., Drexler, Wolfgang, Schmetterer, Leopold, Pramanik, Manojit
Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
Format: Article
Language:English
Published: 2024
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Online Access:https://hdl.handle.net/10356/173924
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1739242024-03-08T15:31:44Z Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye Haindl, Richard Bellemo, Valentina Rajendran, Praveenbalaji Tan, Bingyao Liu, Mengyang Lee, Beishi Zhou, Qifa Leitgeb, Rainer A. Drexler, Wolfgang Schmetterer, Leopold Pramanik, Manojit School of Chemistry, Chemical Engineering and Biotechnology Lee Kong Chian School of Medicine (LKCMedicine) Singapore National Eye Centre Duke-NUS Medical School SERI-NTU Advanced Ocular Engineering (STANCE) Program Engineering Non-invasive imaging Visible light Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, and permissible light fluence at the retina. This study introduces an innovative approach that utilizes Rose Bengal, a contrast agent, to enhance PAOM contrast. This enables visualization of deeper structures, such as the choroidal vasculature and sclera in the mouse eye, using visible light. The integration of near-infrared-II (NIR-II) optical coherence tomography provides additional tissue contrast and insights into potential NIR-II PAOM capabilities. To optimize imaging, we developed a cost-effective 3D printable mouse eye phantom and a fully 3D printable tip/tilt mouse platform. This solution elevates PAOM to a user-friendly technology, which can be used to address pressing research questions concerning several ocular diseases, such as myopia, glaucoma, and/or age-related macular degeneration in the future. Agency for Science, Technology and Research (A*STAR) Nanyang Technological University National Medical Research Council (NMRC) National Research Foundation (NRF) Published version This work was funded by grants from the National Medical Research Council (Grant Nos. CG/C010A/2017-SERI, OFLCG/004c/2018-00, MOH-000249-00, MOH-000647-00, MOH-001001-00, MOH-001015-00, MOH-000500-00, MOH-000707-00, MOH-001072-06, and MOH-001286-00), the National Research Foundation Singapore (Grant Nos. NRF2019-THE002-0006 and NRF-CRP24-2020-0001), ASTAR (Grant No. A20H4b0141), the Singapore Eye Research Institute and Nanyang Technological University [SERI-NTU Advanced Ocular Engineering (STANCE) Program], and the SERI-Lee Foundation (Grant No. LF1019-1), Singapore. M. Liu is funded by the H2020-MSCA-IF-2019 project SkinOptima with Grant Agreement No. 894325. 2024-03-06T05:52:35Z 2024-03-06T05:52:35Z 2023 Journal Article Haindl, R., Bellemo, V., Rajendran, P., Tan, B., Liu, M., Lee, B., Zhou, Q., Leitgeb, R. A., Drexler, W., Schmetterer, L. & Pramanik, M. (2023). Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye. APL Photonics, 8(10), 106108-1-106108-11. https://dx.doi.org/10.1063/5.0168091 2378-0967 https://hdl.handle.net/10356/173924 10.1063/5.0168091 2-s2.0-85175810825 10 8 106108-1 106108-11 en CG/C010A/2017-SERI OFLCG/004c/2018-00 MOH-000249-00 MOH-000647-00 MOH-001001-00 MOH-001015-00 MOH-000500-00 MOH-000707-00 MOH-001072-06 MOH-001286-00 NRF2019-THE002-0006 NRF-CRP24-2020-0001 A20H4b0141 LF1019-1 APL Photonics © 2023 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering
Non-invasive imaging
Visible light
spellingShingle Engineering
Non-invasive imaging
Visible light
Haindl, Richard
Bellemo, Valentina
Rajendran, Praveenbalaji
Tan, Bingyao
Liu, Mengyang
Lee, Beishi
Zhou, Qifa
Leitgeb, Rainer A.
Drexler, Wolfgang
Schmetterer, Leopold
Pramanik, Manojit
Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye
description Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, and permissible light fluence at the retina. This study introduces an innovative approach that utilizes Rose Bengal, a contrast agent, to enhance PAOM contrast. This enables visualization of deeper structures, such as the choroidal vasculature and sclera in the mouse eye, using visible light. The integration of near-infrared-II (NIR-II) optical coherence tomography provides additional tissue contrast and insights into potential NIR-II PAOM capabilities. To optimize imaging, we developed a cost-effective 3D printable mouse eye phantom and a fully 3D printable tip/tilt mouse platform. This solution elevates PAOM to a user-friendly technology, which can be used to address pressing research questions concerning several ocular diseases, such as myopia, glaucoma, and/or age-related macular degeneration in the future.
author2 School of Chemistry, Chemical Engineering and Biotechnology
author_facet School of Chemistry, Chemical Engineering and Biotechnology
Haindl, Richard
Bellemo, Valentina
Rajendran, Praveenbalaji
Tan, Bingyao
Liu, Mengyang
Lee, Beishi
Zhou, Qifa
Leitgeb, Rainer A.
Drexler, Wolfgang
Schmetterer, Leopold
Pramanik, Manojit
format Article
author Haindl, Richard
Bellemo, Valentina
Rajendran, Praveenbalaji
Tan, Bingyao
Liu, Mengyang
Lee, Beishi
Zhou, Qifa
Leitgeb, Rainer A.
Drexler, Wolfgang
Schmetterer, Leopold
Pramanik, Manojit
author_sort Haindl, Richard
title Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye
title_short Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye
title_full Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye
title_fullStr Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye
title_full_unstemmed Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye
title_sort visible light photoacoustic ophthalmoscopy and near-infrared-ii optical coherence tomography in the mouse eye
publishDate 2024
url https://hdl.handle.net/10356/173924
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