Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging

Bacterial infections remain as a prevailing situation in our today’s society. Owing to the evolving pathogenicity of bacteria, whereby more drug-resistant bacteria has been emerging and bacteria developing their own defense mechanism, traditional antimicrobial approaches like antibiotics treatment h...

Full description

Saved in:
Bibliographic Details
Main Author: Yan, Evelias Hui Xin
Other Authors: Xing Bengang
Format: Thesis-Master by Research
Language:English
Published: Nanyang Technological University 2024
Subjects:
Online Access:https://hdl.handle.net/10356/173983
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-173983
record_format dspace
spelling sg-ntu-dr.10356-1739832024-04-09T03:58:58Z Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging Yan, Evelias Hui Xin Xing Bengang School of Chemistry, Chemical Engineering and Biotechnology Bengang@ntu.edu.sg Chemistry Fluorescent imaging Bacterial infections Small molecules Enzyme-responsive Bacterial infections remain as a prevailing situation in our today’s society. Owing to the evolving pathogenicity of bacteria, whereby more drug-resistant bacteria has been emerging and bacteria developing their own defense mechanism, traditional antimicrobial approaches like antibiotics treatment have been clinically ineffective. Early and accurate strategies are off dire need to subvert the rapid colonization of bacteria. Hence, the employment of fluorescence imaging for specific bacterial labeling and infection diagnosis could be prospective in providing physiological information in real time for the diagnosis and therapeutic intervention of bacterial infections. Small-molecule fluorescence probes have been an attractive tool in fluorescence imaging due to their inherent properties such as highly tunable emissions, high quantum yield and good biocompatibility. Among the numerous types of small-molecule fluorescence probes, the enzymatic responsive probes have been one that researchers are particularly interested in as such probes were reported to possess great specificity and high signal to noise ratio. Furthermore, bacterial enzymes are found to be closely related to the invasion of host immune system, which makes them a viable target to establish enzyme-activated fluorescent probes for specific bacterial imaging. In chapter 1, we will be giving a brief background on bacterial infections, introducing the principles and techniques used in the design of small-molecule fluorescence probes, and followed by providing some of the reported examples of the recent and typical strategies for enzyme-activated small-molecule fluorescence probes. In chapter 2, we explore the specific proteolytic activity of an outer membrane bacterial protease that is present in all wild-type Escherichia coli (E. coli) and another bacterial protease located in the periplasmic region of E. coli to synthesize a near infrared fluorescence probe for the real-time imaging of urinary tract bacterial infection in vitro and in vivo. In chapter 3, the experimental section and supplementary figures are presented. Master's degree 2024-03-11T07:41:34Z 2024-03-11T07:41:34Z 2023 Thesis-Master by Research Yan, E. H. X. (2023). Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging. Master's thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/173983 https://hdl.handle.net/10356/173983 10.32657/10356/173983 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Chemistry
Fluorescent imaging
Bacterial infections
Small molecules
Enzyme-responsive
spellingShingle Chemistry
Fluorescent imaging
Bacterial infections
Small molecules
Enzyme-responsive
Yan, Evelias Hui Xin
Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
description Bacterial infections remain as a prevailing situation in our today’s society. Owing to the evolving pathogenicity of bacteria, whereby more drug-resistant bacteria has been emerging and bacteria developing their own defense mechanism, traditional antimicrobial approaches like antibiotics treatment have been clinically ineffective. Early and accurate strategies are off dire need to subvert the rapid colonization of bacteria. Hence, the employment of fluorescence imaging for specific bacterial labeling and infection diagnosis could be prospective in providing physiological information in real time for the diagnosis and therapeutic intervention of bacterial infections. Small-molecule fluorescence probes have been an attractive tool in fluorescence imaging due to their inherent properties such as highly tunable emissions, high quantum yield and good biocompatibility. Among the numerous types of small-molecule fluorescence probes, the enzymatic responsive probes have been one that researchers are particularly interested in as such probes were reported to possess great specificity and high signal to noise ratio. Furthermore, bacterial enzymes are found to be closely related to the invasion of host immune system, which makes them a viable target to establish enzyme-activated fluorescent probes for specific bacterial imaging. In chapter 1, we will be giving a brief background on bacterial infections, introducing the principles and techniques used in the design of small-molecule fluorescence probes, and followed by providing some of the reported examples of the recent and typical strategies for enzyme-activated small-molecule fluorescence probes. In chapter 2, we explore the specific proteolytic activity of an outer membrane bacterial protease that is present in all wild-type Escherichia coli (E. coli) and another bacterial protease located in the periplasmic region of E. coli to synthesize a near infrared fluorescence probe for the real-time imaging of urinary tract bacterial infection in vitro and in vivo. In chapter 3, the experimental section and supplementary figures are presented.
author2 Xing Bengang
author_facet Xing Bengang
Yan, Evelias Hui Xin
format Thesis-Master by Research
author Yan, Evelias Hui Xin
author_sort Yan, Evelias Hui Xin
title Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
title_short Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
title_full Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
title_fullStr Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
title_full_unstemmed Small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
title_sort small but prospective: development of enzyme-responsive small molecule fluorescent probes for specific bacterial imaging
publisher Nanyang Technological University
publishDate 2024
url https://hdl.handle.net/10356/173983
_version_ 1814047176646459392