Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches

• Main Authors: Sana, Barindra, Ding, Ke, Siau, Jia Wei, Pasula, Rupali Reddy, Chee, Sharon, Kharel, Sharad, Lena, Jean-Baptise Henri, Goh, Eunice, Rajamani, Lakshminarayanan, Lam, Yeng Ming, Lim, Sierin, Ghadessy, John F.
• Other Authors: School of Materials Science and Engineering
• Format: Article
• Language: English
• Published: 2024
• Subjects: Engineering; Hydrolase; Thermostability
• Online Access: https://hdl.handle.net/10356/174342
• Institution: Nanyang Technological University

Polyethylene terephthalate (PET) hydrolase enzymes show promise for enzymatic PET degradation and green recycling of single-use PET vessels representing a major source of global pollution. Their full potential can be unlocked with enzyme engineering to render activities on recalcitrant PET substrate...