NAPE-PLD, a viable antimalarial drug target?
The emergence of artemisinin-resistant strain of Plasmodium falciparum worsens global outlook of malaria as it impedes on the elimination of the disease. Due to added resistance, ring-stage parasite are becoming harder to eliminate, therefore exacerbating the need for novel ring-stage target for ant...
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| Format: | Final Year Project |
| Language: | English |
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Nanyang Technological University
2024
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| Online Access: | https://hdl.handle.net/10356/176529 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The emergence of artemisinin-resistant strain of Plasmodium falciparum worsens global outlook of malaria as it impedes on the elimination of the disease. Due to added resistance, ring-stage parasite are becoming harder to eliminate, therefore exacerbating the need for novel ring-stage target for antimalarial treatments.In this study, we investigated N-acylphosphatidylethanolamine Phospholipase D (NAPEPLD) as a potential ring-stage target for treatment. It was found that the known inhibitors of NAPE-PLD, LEI-401 completely inhibited the parasite at the schizont and early ring stages, while ARN-19874 only partially inhibits. It was also revealed that NAPE-PLD may follow the PEXEL export pathway, as immunofluorescence assay suggests localisation in the parasitophorous vacuole membrane (PVM). Overall, the high IC50 of LEI-401 disqualifies it as an antimalarial treatment but may retain utility in elucidation of new drug targets. |
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