Programmable liquid patterning device for skin culture and research

Skin grafting is a process that transfers skin from one part of the body to another part that has lost the protective covering of skin. This process is commonly used to treat third-degree burns. Before skin grafting can be performed, new skin needs to be created. One of the main components of skin g...

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Main Author: Soetarto, Andrew Darren
Other Authors: Marcos
Format: Final Year Project
Language:English
Published: Nanyang Technological University 2024
Subjects:
Online Access:https://hdl.handle.net/10356/176800
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1768002024-05-25T16:49:46Z Programmable liquid patterning device for skin culture and research Soetarto, Andrew Darren Marcos School of Mechanical and Aerospace Engineering marcos@ntu.edu.sg Engineering Fibrin Skin grafting is a process that transfers skin from one part of the body to another part that has lost the protective covering of skin. This process is commonly used to treat third-degree burns. Before skin grafting can be performed, new skin needs to be created. One of the main components of skin graft preparation is scaffold creation to facilitate the growth of skin cells. Fibrin has been proven to have numerous advantages as scaffold for skin grafting. The conventional way to prepare fibrin scaffold is to manually extrude fibrin using a double syringe system in a zig-zag pattern along a flat surface. This Final Year Project (FYP) is a continuation of a previous FYP. A compact machine was assembled in the previous FYP to automate the process of dispensing liquid onto a 10 x 10 cm2 square petri dish. However, due to time and cost limitations in the previous project, deposition of fibrin onto a petri dish was not done. In this FYP, optimizations and machine functions creation were done. A framework was created to calibrate the machine with different liquids through various tests. These tests are used to determine the essential dispensing parameters: bed speed, extrude width, and extruder speed. The machine is capable of producing an even surface of both water and agar on the square petri dish using less than 5 mL of liquid. Afterwards, experiments were done to determine the optimal machine parameters to produce an even fibrin scaffold. Multiple experiments were conducted using fibrin. Experiments were done to determine the values of the three dispensing parameters for fibrin. The scaffold sheets produced in the tests were not uniform, leaving few gaps. The effects of the dispensing parameters on fibrin were discussed. Finally, the petri dish was treated with plasma cleaner to change the surface of the dish from hydrophobic to hydrophilic to promote better adhesion of fibrin onto the surface. This showed promising results as it induced line-to-line self-assembly of fibrin, even though there were errors. Lastly, limitations and future works are discussed. Bachelor's degree 2024-05-20T04:09:19Z 2024-05-20T04:09:19Z 2024 Final Year Project (FYP) Soetarto, A. D. (2024). Programmable liquid patterning device for skin culture and research. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/176800 https://hdl.handle.net/10356/176800 en application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering
Fibrin
spellingShingle Engineering
Fibrin
Soetarto, Andrew Darren
Programmable liquid patterning device for skin culture and research
description Skin grafting is a process that transfers skin from one part of the body to another part that has lost the protective covering of skin. This process is commonly used to treat third-degree burns. Before skin grafting can be performed, new skin needs to be created. One of the main components of skin graft preparation is scaffold creation to facilitate the growth of skin cells. Fibrin has been proven to have numerous advantages as scaffold for skin grafting. The conventional way to prepare fibrin scaffold is to manually extrude fibrin using a double syringe system in a zig-zag pattern along a flat surface. This Final Year Project (FYP) is a continuation of a previous FYP. A compact machine was assembled in the previous FYP to automate the process of dispensing liquid onto a 10 x 10 cm2 square petri dish. However, due to time and cost limitations in the previous project, deposition of fibrin onto a petri dish was not done. In this FYP, optimizations and machine functions creation were done. A framework was created to calibrate the machine with different liquids through various tests. These tests are used to determine the essential dispensing parameters: bed speed, extrude width, and extruder speed. The machine is capable of producing an even surface of both water and agar on the square petri dish using less than 5 mL of liquid. Afterwards, experiments were done to determine the optimal machine parameters to produce an even fibrin scaffold. Multiple experiments were conducted using fibrin. Experiments were done to determine the values of the three dispensing parameters for fibrin. The scaffold sheets produced in the tests were not uniform, leaving few gaps. The effects of the dispensing parameters on fibrin were discussed. Finally, the petri dish was treated with plasma cleaner to change the surface of the dish from hydrophobic to hydrophilic to promote better adhesion of fibrin onto the surface. This showed promising results as it induced line-to-line self-assembly of fibrin, even though there were errors. Lastly, limitations and future works are discussed.
author2 Marcos
author_facet Marcos
Soetarto, Andrew Darren
format Final Year Project
author Soetarto, Andrew Darren
author_sort Soetarto, Andrew Darren
title Programmable liquid patterning device for skin culture and research
title_short Programmable liquid patterning device for skin culture and research
title_full Programmable liquid patterning device for skin culture and research
title_fullStr Programmable liquid patterning device for skin culture and research
title_full_unstemmed Programmable liquid patterning device for skin culture and research
title_sort programmable liquid patterning device for skin culture and research
publisher Nanyang Technological University
publishDate 2024
url https://hdl.handle.net/10356/176800
_version_ 1800916104629977088