Low-cost incubator for live-cell imaging

• Main Author: Goh, Rui Si
• Other Authors: Tong Ling
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2024
• Subjects: Engineering; Live-cell imaging; Spiking human embryonic cells and neuron cells; Incubator
• Online Access: https://hdl.handle.net/10356/177181
• Institution: Nanyang Technological University

Electrophysiology is an application of live-cell imaging which measures the electrical activity of cells. This application requires effective temperature control at 37 ℃ for successful experiments. This report discusses how an incubator is designed in terms of its hardware and software. In this project the target microscope is the Nikon Ti-2-U microscope. The incubator is designed to have an effective temperature control that maintains the environment in the incubator at 37 ℃, as well as to ensure its user friendliness. The report then talks about how the incubator is then tested to determine the effectiveness of the incubator with neuron cells and spiking human embryonic cells (SHEK cells).