Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production

The global energy crisis has intensified the search for sustainable and clean alternatives, with biohydrogen emerging as a promising solution to address environmental challenges. Leveraging photo fermentation (PF) process, purple phototrophic bacteria (PPB) can harness reducing power derived from or...

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Main Authors: Huang, Peitian, Chen, Yun, Li, Zong, Zhang, Baorui, Yu, Siwei, Zhou, Yan
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2024
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Online Access:https://hdl.handle.net/10356/179279
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-1792792024-07-24T05:22:18Z Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production Huang, Peitian Chen, Yun Li, Zong Zhang, Baorui Yu, Siwei Zhou, Yan School of Civil and Environmental Engineering Interdisciplinary Graduate School (IGS) Nanyang Environment and Water Research Institute Engineering Purple non-sulfur bacteria Photosynthetic bacteria The global energy crisis has intensified the search for sustainable and clean alternatives, with biohydrogen emerging as a promising solution to address environmental challenges. Leveraging photo fermentation (PF) process, purple phototrophic bacteria (PPB) can harness reducing power derived from organic substrates to facilitate hydrogen production. However, existing studies report much lower H2 yields than theoretical value when using acetate as carbon source and ammonia as nitrogen source, primarily attributed to the widely employed pulse-feeding mode which suffers from ammonia inhibition effect on nitrogenase. To address this issue, a continuous feeding mode was applied to avoid ammonia accumulation in this study. On the other hand, other pathways like carbon fixation and polyhydroxyalkanoate (PHA) formation could compete reducing power with H2 production. However, the reducing power allocation under continuous feeding mode is not yet clear. In this study, the reducing power allocation and hydrogen production performance were evaluated under various ammonia loading, using acetate as carbon source and infrared LED at around 50 W·m-2 as light source. The results show that (a) The absence of ammonia resulted in the best performance for hydrogen production, with 44 % of the reducing power distributed to H2 and the highest H2 volumetric productivity, while the allocation of reducing power to hydrogen production stopped when ammonia loading was above 7.6 mg NH4-N·L-1·d-1; (b) when PPB required to eliminate reducing power under ammonia limited conditions, PHA production was the preferred pathway followed by the hydrogen production pathway, but once PHA accumulation reached saturation, hydrogen generation pathway dominated; (c) under ammonia limited conditions, the TCA cycle was more activated rendering higher NADH (i.e. reducing power) production compared with that under ammonia sufficient conditions which was verified by metagenomics analysis, and all the hydrogen production, PHA accumulation and carbon fixation pathways were highly active to dissipate reducing power. This work provides the insight of reducing power distribution and PPB biohydrogen production variated by ammonia loading under continuous feeding mode. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) Nanyang Technological University The authors would like to thank the Nanyang Environment & Water Research Institute and the Interdisciplinary Graduate Programme, Nanyang Technological University, Singapore, for the award of research scholarship. This work was supported by A*STAR SFS IAF-PP grant (A20H7a0152) and AcRF Tier 1 RT 12/20 awarded to Yan Zhou. 2024-07-24T05:22:18Z 2024-07-24T05:22:18Z 2024 Journal Article Huang, P., Chen, Y., Li, Z., Zhang, B., Yu, S. & Zhou, Y. (2024). Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production. Water Research, 256, 121599-. https://dx.doi.org/10.1016/j.watres.2024.121599 0043-1354 https://hdl.handle.net/10356/179279 10.1016/j.watres.2024.121599 38615602 2-s2.0-85190245129 256 121599 en A20H7a0152 RT 12/20 Water Research © 2024 Elsevier Ltd. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering
Purple non-sulfur bacteria
Photosynthetic bacteria
spellingShingle Engineering
Purple non-sulfur bacteria
Photosynthetic bacteria
Huang, Peitian
Chen, Yun
Li, Zong
Zhang, Baorui
Yu, Siwei
Zhou, Yan
Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
description The global energy crisis has intensified the search for sustainable and clean alternatives, with biohydrogen emerging as a promising solution to address environmental challenges. Leveraging photo fermentation (PF) process, purple phototrophic bacteria (PPB) can harness reducing power derived from organic substrates to facilitate hydrogen production. However, existing studies report much lower H2 yields than theoretical value when using acetate as carbon source and ammonia as nitrogen source, primarily attributed to the widely employed pulse-feeding mode which suffers from ammonia inhibition effect on nitrogenase. To address this issue, a continuous feeding mode was applied to avoid ammonia accumulation in this study. On the other hand, other pathways like carbon fixation and polyhydroxyalkanoate (PHA) formation could compete reducing power with H2 production. However, the reducing power allocation under continuous feeding mode is not yet clear. In this study, the reducing power allocation and hydrogen production performance were evaluated under various ammonia loading, using acetate as carbon source and infrared LED at around 50 W·m-2 as light source. The results show that (a) The absence of ammonia resulted in the best performance for hydrogen production, with 44 % of the reducing power distributed to H2 and the highest H2 volumetric productivity, while the allocation of reducing power to hydrogen production stopped when ammonia loading was above 7.6 mg NH4-N·L-1·d-1; (b) when PPB required to eliminate reducing power under ammonia limited conditions, PHA production was the preferred pathway followed by the hydrogen production pathway, but once PHA accumulation reached saturation, hydrogen generation pathway dominated; (c) under ammonia limited conditions, the TCA cycle was more activated rendering higher NADH (i.e. reducing power) production compared with that under ammonia sufficient conditions which was verified by metagenomics analysis, and all the hydrogen production, PHA accumulation and carbon fixation pathways were highly active to dissipate reducing power. This work provides the insight of reducing power distribution and PPB biohydrogen production variated by ammonia loading under continuous feeding mode.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Huang, Peitian
Chen, Yun
Li, Zong
Zhang, Baorui
Yu, Siwei
Zhou, Yan
format Article
author Huang, Peitian
Chen, Yun
Li, Zong
Zhang, Baorui
Yu, Siwei
Zhou, Yan
author_sort Huang, Peitian
title Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
title_short Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
title_full Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
title_fullStr Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
title_full_unstemmed Ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
title_sort ammonia-dependent reducing power redistribution for purple phototrophic bacteria culture-based biohydrogen production
publishDate 2024
url https://hdl.handle.net/10356/179279
_version_ 1814047384788795392