Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media

Saccharification serves as the entry point for the biomass into biorefinery schemes and plays a vital role in lignocellulose transformation towards bio-fuels and platform molecules. Herein, an efficient in situ saccharification of lignocellulose under the mediation of ionic liquid of [DMIM]DMP is re...

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Main Authors: Lei, Junxi, Deng, Limin, Chen, Zongda, Li, Xiang, Li, Kaixin, Lee, Jong-Min
Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
Format: Article
Language:English
Published: 2024
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Online Access:https://hdl.handle.net/10356/180229
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1802292024-09-25T01:23:07Z Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media Lei, Junxi Deng, Limin Chen, Zongda Li, Xiang Li, Kaixin Lee, Jong-Min School of Chemistry, Chemical Engineering and Biotechnology Engineering Biomass waste Saccharification Saccharification serves as the entry point for the biomass into biorefinery schemes and plays a vital role in lignocellulose transformation towards bio-fuels and platform molecules. Herein, an efficient in situ saccharification of lignocellulose under the mediation of ionic liquid of [DMIM]DMP is reported, using a series of magnetically-separable biochars as catalyst supports with immobilized endoglucanase Cel5A. The physicochemical properties of these magnetic biochars with immobilized endoglucanase (abbreviated as MBIE) were thoroughly characterized by FTIR, TG, SEM/EDX, XRD, XPS, N2 physisorption, UV–vis and VSM. The results revealed that the endoglucanase, which functions as the catalytically active species, was immobilized on the biochar via simultaneous physical adsorption and covalent attachment. The extremely high surface area of biochar endows a high loading of endoglucanase and easy diffusion of reactant within the catalyst's framework. The zeta potential of the catalyst was − 7.06 mV, indicating its excellent stability. MBIE with immobilizing endoglucanase of 484.36 mg/g exhibited the superior performance under a biphasic system (IL/water v/v 5:1), affording a glucose yield of 932.5 mg/g. The activity of MBIE remained constant after 10 consecutive cycles via magnetic recycling. This work demonstrates an efficient in situ saccharification process catalyzed by the developed MBIE with high activity and excellent regeneration ability. This work was financially supported by the Science and Technology Planning Project of Guangdong Province of China (2019A050510014), the Natural Science Foundation of Guangdong Province of China (2023A1515011751) and the Science and Technology Planning Project of Guangzhou city (2023B03J1285). 2024-09-25T01:23:07Z 2024-09-25T01:23:07Z 2024 Journal Article Lei, J., Deng, L., Chen, Z., Li, X., Li, K. & Lee, J. (2024). Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media. Chemical Engineering Journal, 485, 149990-. https://dx.doi.org/10.1016/j.cej.2024.149990 1385-8947 https://hdl.handle.net/10356/180229 10.1016/j.cej.2024.149990 2-s2.0-85186717356 485 149990 en Chemical Engineering Journal © 2024 Elsevier B.V. All rights reserved.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering
Biomass waste
Saccharification
spellingShingle Engineering
Biomass waste
Saccharification
Lei, Junxi
Deng, Limin
Chen, Zongda
Li, Xiang
Li, Kaixin
Lee, Jong-Min
Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
description Saccharification serves as the entry point for the biomass into biorefinery schemes and plays a vital role in lignocellulose transformation towards bio-fuels and platform molecules. Herein, an efficient in situ saccharification of lignocellulose under the mediation of ionic liquid of [DMIM]DMP is reported, using a series of magnetically-separable biochars as catalyst supports with immobilized endoglucanase Cel5A. The physicochemical properties of these magnetic biochars with immobilized endoglucanase (abbreviated as MBIE) were thoroughly characterized by FTIR, TG, SEM/EDX, XRD, XPS, N2 physisorption, UV–vis and VSM. The results revealed that the endoglucanase, which functions as the catalytically active species, was immobilized on the biochar via simultaneous physical adsorption and covalent attachment. The extremely high surface area of biochar endows a high loading of endoglucanase and easy diffusion of reactant within the catalyst's framework. The zeta potential of the catalyst was − 7.06 mV, indicating its excellent stability. MBIE with immobilizing endoglucanase of 484.36 mg/g exhibited the superior performance under a biphasic system (IL/water v/v 5:1), affording a glucose yield of 932.5 mg/g. The activity of MBIE remained constant after 10 consecutive cycles via magnetic recycling. This work demonstrates an efficient in situ saccharification process catalyzed by the developed MBIE with high activity and excellent regeneration ability.
author2 School of Chemistry, Chemical Engineering and Biotechnology
author_facet School of Chemistry, Chemical Engineering and Biotechnology
Lei, Junxi
Deng, Limin
Chen, Zongda
Li, Xiang
Li, Kaixin
Lee, Jong-Min
format Article
author Lei, Junxi
Deng, Limin
Chen, Zongda
Li, Xiang
Li, Kaixin
Lee, Jong-Min
author_sort Lei, Junxi
title Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
title_short Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
title_full Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
title_fullStr Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
title_full_unstemmed Efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
title_sort efficient in situ saccharification of microcrystalline cellulose over immobilized cellulase on magnetic biochar in ionic liquid media
publishDate 2024
url https://hdl.handle.net/10356/180229
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