Sub-micrometer electrospray ionization emitter enables direct infusion native mass spectrometric analysis of proteins and protein-ligand complexes in NaCl, Tris-HCl buffer

• Main Author: Lim, Su Wei
• Other Authors: Xueming Dong
• Format: Final Year Project
• Language: English
• Published: Nanyang Technological University 2024
• Subjects: Medicine, Health and Life Sciences; Native mass spectrometry; Sub-micrometer emitters
• Online Access: https://hdl.handle.net/10356/181012
• Institution: Nanyang Technological University

In conventional electrospray ionisation (ESI) native mass spectrometry (native MS), proteins and protein-ligand complexes can be identified in minute amounts in a matter of minutes. To stabilize the native structures of proteins and protein-ligand complexes during analysis, salts and non-volat...