A circularly permuted CasRx platform for efficient, site-specific RNA editing

• Main Authors: Wang, Yuanming, Liu, Ivy Kaiwen, Liu, Mandy Mengying, Ooi, Kean Hean, Nguyen, Tram Anh, Chee, Jiunn En, Teo, Danny Shun Xiang, He, Shan, Tay, Douglas Jie Wen, Teo, Seok Yee, Liew, Kai Shin, Ge, Xiao Yu, Ng, Zhi Jian, Avagyan, Hasmik, Liu, Hao, Yi, Zirong, Chang, Keziah, Kok, Louis Eng Piew, Chen, Runjia, Yau, Chun En, Koh, Jun Wei, Wan, Yue, Tan, Meng How
• Other Authors: School of Chemistry, Chemical Engineering and Biotechnology
• Format: Article
• Language: English
• Published: 2025
• Subjects: Chemistry; RNA editing; Biochemical engineering
• Online Access: https://hdl.handle.net/10356/181953
• Institution: Nanyang Technological University

Inactive Cas13 orthologs have been fused to a mutant human ADAR2 deaminase domain at the C terminus to enable programmable adenosine-to-inosine (A-to-I) RNA editing in selected transcripts. Although promising, existing RNA-editing tools generally suffer from a trade-off between efficacy and specific...