Non-destructive viability assessment of cancer cell spheroids using dynamic optical coherence tomography with trypan blue validation

• Main Authors: Tan, Ko Hui, Ang, Joel Lang Yi, Yong, Alexander Si Kai, Lim, Stefanie Zi En, Kng, Jessica Sze Jia, Liang, Kaicheng
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2025
• Subjects: Medicine, Health and Life Sciences; Cancer cell; Optical coherence tomography
• Online Access: https://hdl.handle.net/10356/182111
• Institution: Nanyang Technological University

3D cell cultures are widely used in biomedical research for the recapitulation of in vivo microenvironments. Viability assessment and monitoring of these intricate conformations remain an open problem as standard cell viability protocols based on colorimetry or microscopy are not directly applicable...