Genetic studies of tropical ornamental fish

The discovery that the use of PCR with an arbitrarily selected primer to amplify a specific set of arbitrarily distributed loci in any genome laid the foundation for high output of genetic markers that can be used for a variety of purposes. In this study, RAPD was utilized as a method in the identif...

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Bibliographic Details
Main Author: Lim, Chee Whye.
Other Authors: National Institute of Education
Format: Theses and Dissertations
Language:English
Published: 2009
Subjects:
Online Access:http://hdl.handle.net/10356/18885
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Institution: Nanyang Technological University
Language: English
Description
Summary:The discovery that the use of PCR with an arbitrarily selected primer to amplify a specific set of arbitrarily distributed loci in any genome laid the foundation for high output of genetic markers that can be used for a variety of purposes. In this study, RAPD was utilized as a method in the identification, interrelationship analysis and the construction of a basic linkage map of medaka (Oryzias latipes). The optimized RAPD-PCR protocol obtained, reveal a moderate degree of genetic diversity among the strains of medaka studied.