Targeted therapy to gastric cancer
Gastric cancer is a leading cause of death in Asia and a heterogeneous disease. The aim of targeted therapy is to specifically induce cancer cells apoptosis with minimum side effects on normal cells. In this study, 17 gastric cancer cell lines were used to screen a library of 60 small molecule in...
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sg-ntu-dr.10356-190532023-02-28T18:00:40Z Targeted therapy to gastric cancer Hou, Qian. Sze Siu Kwan School of Biological Sciences DRNTU::Science::Medicine Gastric cancer is a leading cause of death in Asia and a heterogeneous disease. The aim of targeted therapy is to specifically induce cancer cells apoptosis with minimum side effects on normal cells. In this study, 17 gastric cancer cell lines were used to screen a library of 60 small molecule inhibitors and chemotherapy drugs for discovery of potential therapeutic agent by methyl thiazole tetrazolium (MTT) assays. 8 effective drugs which include Docetaxel, Paclitaxel, 17-DMAG, Bortezomib, Calyculin A, Okadaic acid, Staurosporine and Bafilomycin A1 were identified and subject to further characterization by IC50 calculation, cell migration assay, morphology study and cell cycle analysis by FACS. The 8 small molecule compounds were shown to be effective in inhibiting gastric cancer cell growth at nanomolar concentrations in vitro. These drugs’ targets: HSP90, proteasome, protein phosphatase and V-ATPase provide insights for drug design trend for gastric cancer therapy. Unlike drugs that target specific kinases, the 8 drugs identified here indirectly inhibit multiple signaling pathways which are essential for cancer cell survival. As cancer is a heterogeneous disease with multiple aberrant signaling pathways, inhibiting these targets simultaneously may be an effective therapy for gastric cancer. Bachelor of Science in Biological Sciences 2009-09-14T06:43:06Z 2009-09-14T06:43:06Z 2009 2009 Final Year Project (FYP) http://hdl.handle.net/10356/19053 en Nanyang Technological University 44 p. application/pdf |
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DRNTU::Science::Medicine Hou, Qian. Targeted therapy to gastric cancer |
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Gastric cancer is a leading cause of death in Asia and a heterogeneous disease. The
aim of targeted therapy is to specifically induce cancer cells apoptosis with minimum
side effects on normal cells. In this study, 17 gastric cancer cell lines were used to
screen a library of 60 small molecule inhibitors and chemotherapy drugs for discovery of potential therapeutic agent by methyl thiazole tetrazolium (MTT) assays. 8 effective drugs which include Docetaxel, Paclitaxel, 17-DMAG, Bortezomib, Calyculin A, Okadaic acid, Staurosporine and Bafilomycin A1 were identified and subject to further characterization by IC50 calculation, cell migration assay, morphology study and cell cycle analysis by FACS. The 8 small molecule compounds were shown to be effective in inhibiting gastric cancer cell growth at nanomolar concentrations in vitro. These drugs’ targets: HSP90, proteasome, protein phosphatase and V-ATPase provide insights for drug design trend for gastric cancer therapy. Unlike drugs that target specific
kinases, the 8 drugs identified here indirectly inhibit multiple signaling pathways which are essential for cancer cell survival. As cancer is a heterogeneous disease with multiple aberrant signaling pathways, inhibiting these targets simultaneously may be an effective therapy for gastric cancer. |
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Sze Siu Kwan |
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Sze Siu Kwan Hou, Qian. |
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Final Year Project |
author |
Hou, Qian. |
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Hou, Qian. |
title |
Targeted therapy to gastric cancer |
title_short |
Targeted therapy to gastric cancer |
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Targeted therapy to gastric cancer |
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Targeted therapy to gastric cancer |
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Targeted therapy to gastric cancer |
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targeted therapy to gastric cancer |
publishDate |
2009 |
url |
http://hdl.handle.net/10356/19053 |
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1759858000404676608 |