Genetic studies of tropical ornamental fish using RAPD-PCR technology
RAPD-PCR is a useful new molecular technique to characterize and study genomes. In this study, in order to ensure the repeatability and reproducibility of RAPD-PCR results within and between laboratories, various factors influencing the reproducibility of amplification were investigated systematical...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/10356/20279 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | RAPD-PCR is a useful new molecular technique to characterize and study genomes. In this study, in order to ensure the repeatability and reproducibility of RAPD-PCR results within and between laboratories, various factors influencing the reproducibility of amplification were investigated systematically. Results indicate that template DNA and magnesium factors were important parameters which affected reproducibility. Variation in these factors could cause discrepancies in amplification of certain RAPD bands. It was important to use relatively pure DNA samples for RAPD-PCR amplifications. Although a standard protocol for genomic DNA extraction was employed, the purity of DNA extracts varied from sample to sample. Impure DNA samples had to be re-purified before they were used for amplifications. |
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